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dc.contributor.author Cabrera Mederos, Dariel
dc.contributor.author Dal Zotto, A.
dc.contributor.author Galdeano, Ernestina
dc.contributor.author Portal, Orelvis
dc.contributor.author Giolitti, Fabián José
dc.date.available 2017-08-17T21:21:32Z
dc.date.issued 2016-12
dc.identifier.citation Cabrera Mederos, Dariel; Dal Zotto, A.; Galdeano, Ernestina; Portal, Orelvis; Giolitti, Fabián José; First report of Papaya ringspot virus infecting Carica papaya In Argentina; Edizioni Ets; Journal Of Plant Pathology; 98; 3; 12-2016; 677-697
dc.identifier.issn 1125-4653
dc.identifier.uri http://hdl.handle.net/11336/22641
dc.description.abstract Papaya plants (Carica papaya L.) with severe leaf mosaic and deformation symptoms were observed and collected in the Formosa, Corrientes and Misiones provinces of northern Argentina. These symptoms were similar to those induced by Papaya ringspot virus (PRSV-P) (Tripathi et al., 2008). Electron microscopy observations of leaf dip preparations from symptomatic papaya leaves revealed typical flexuous potyvirus particles of ca. 800×12nm (Francki et al., 1985). Papaya leaf extracts from affected plants were mechanically inoculated on healthy papaya plants, which showed typical symptoms of the disease. The presence of the virus in symptomatic plants was tested and confirmed by double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA), using a specific PRSV antiserum (Agdia, Elkhart, IN). Total RNA was extracted using RNeasy Plant Mini Kit (Qiagen, Hilden, Germany) and tested by reverse transcription-polymerase chain reaction (RT-PCR) with specific PRSV coat protein gene primers: BoCP_Fwd 5’-TCCAAGAATGAAGCTGTGGACGCTGGTT-3’ and BoCP_Rev 5’-TYAGTTGCGCATACCCAGGAGAGAGT-3’. The RT-PCR amplicons of the expected size were purified and directly sequenced in both directions at Macrogen Inc. (Seoul, Korea). BLASTn analysis of the sequenced fragments (828bp) (GenBank accession Nos. KX385113 to KX385116) showed 97 to 98% nucleotide sequence identity with previously reported PRSV-P isolates from Brazil (AF344642, JQ755427, JQ755424). To the best of our knowledge, this is the first report of the occurrence of PRSV in papaya plants in Argentina. Due to the devastating effects of PRSV in papaya (Tripathi et al., 2008) and the recent increase of cultivation area in northern Argentina, it becomes necessary to implement management strategies to control this disease and avoid the introduction of the virus into new production areas.
dc.format application/pdf
dc.language.iso eng
dc.publisher Edizioni Ets
dc.rights info:eu-repo/semantics/openAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject PRSV
dc.subject CARICA PAPAYA
dc.subject VIRUS
dc.subject FIRST REPORT
dc.subject.classification Otras Agricultura, Silvicultura y Pesca
dc.subject.classification Agricultura, Silvicultura y Pesca
dc.subject.classification CIENCIAS AGRÍCOLAS
dc.title First report of Papaya ringspot virus infecting Carica papaya In Argentina
dc.type info:eu-repo/semantics/article
dc.type info:ar-repo/semantics/artículo
dc.type info:eu-repo/semantics/publishedVersion
dc.date.updated 2017-07-13T19:09:47Z
dc.journal.volume 98
dc.journal.number 3
dc.journal.pagination 677-697
dc.journal.pais Italia
dc.journal.ciudad Pisa
dc.description.fil Fil: Cabrera Mederos, Dariel. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil Fil: Dal Zotto, A.. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina
dc.description.fil Fil: Galdeano, Ernestina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
dc.description.fil Fil: Portal, Orelvis. Universidad Central “Marta Abreu” de Las Villas; Cuba
dc.description.fil Fil: Giolitti, Fabián José. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina
dc.journal.title Journal Of Plant Pathology
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.4454/JPP.V98I3.017
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/http://sipav.org/main/jpp/index.php/jpp/article/view/3738


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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)