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dc.contributor.author
Cabrera Mederos, Dariel
dc.contributor.author
Dal Zotto, A.
dc.contributor.author
Galdeano, Ernestina
dc.contributor.author
Portal, Orelvis
dc.contributor.author
Giolitti, Fabián José
dc.date.available
2017-08-17T21:21:32Z
dc.date.issued
2016-12
dc.identifier.citation
Cabrera Mederos, Dariel; Dal Zotto, A.; Galdeano, Ernestina; Portal, Orelvis; Giolitti, Fabián José; First report of Papaya ringspot virus infecting Carica papaya In Argentina; Edizioni Ets; Journal Of Plant Pathology; 98; 3; 12-2016; 677-697
dc.identifier.issn
1125-4653
dc.identifier.uri
http://hdl.handle.net/11336/22641
dc.description.abstract
Papaya plants (Carica papaya L.) with severe leaf mosaic and deformation symptoms were observed and collected in the Formosa, Corrientes and Misiones provinces of northern Argentina. These symptoms were similar to those induced by Papaya ringspot virus (PRSV-P) (Tripathi et al., 2008). Electron microscopy observations of leaf dip preparations from symptomatic papaya leaves revealed typical flexuous potyvirus particles of ca. 800×12nm (Francki et al., 1985). Papaya leaf extracts from affected plants were mechanically inoculated on healthy papaya plants, which showed typical symptoms of the disease. The presence of the virus in symptomatic plants was tested and confirmed by double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA), using a specific PRSV antiserum (Agdia, Elkhart, IN). Total RNA was extracted using RNeasy Plant Mini Kit (Qiagen, Hilden, Germany) and tested by reverse transcription-polymerase chain reaction (RT-PCR) with specific PRSV coat protein gene primers: BoCP_Fwd 5’-TCCAAGAATGAAGCTGTGGACGCTGGTT-3’ and BoCP_Rev 5’-TYAGTTGCGCATACCCAGGAGAGAGT-3’. The RT-PCR amplicons of the expected size were purified and directly sequenced in both directions at Macrogen Inc. (Seoul, Korea). BLASTn analysis of the sequenced fragments (828bp) (GenBank accession Nos. KX385113 to KX385116) showed 97 to 98% nucleotide sequence identity with previously reported PRSV-P isolates from Brazil (AF344642, JQ755427, JQ755424). To the best of our knowledge, this is the first report of the occurrence of PRSV in papaya plants in Argentina. Due to the devastating effects of PRSV in papaya (Tripathi et al., 2008) and the recent increase of cultivation area in northern Argentina, it becomes necessary to implement management strategies to control this disease and avoid the introduction of the virus into new production areas.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Edizioni Ets
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Prsv
dc.subject
Carica Papaya
dc.subject
Virus
dc.subject
First Report
dc.subject.classification
Otras Agricultura, Silvicultura y Pesca
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Agricultura, Silvicultura y Pesca
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CIENCIAS AGRÍCOLAS
dc.title
First report of Papaya ringspot virus infecting Carica papaya In Argentina
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-07-13T19:09:47Z
dc.journal.volume
98
dc.journal.number
3
dc.journal.pagination
677-697
dc.journal.pais
Italia
dc.journal.ciudad
Pisa
dc.description.fil
Fil: Cabrera Mederos, Dariel. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Dal Zotto, A.. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina
dc.description.fil
Fil: Galdeano, Ernestina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
dc.description.fil
Fil: Portal, Orelvis. Universidad Central “Marta Abreu” de Las Villas; Cuba
dc.description.fil
Fil: Giolitti, Fabián José. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina
dc.journal.title
Journal Of Plant Pathology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.4454/JPP.V98I3.017
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://sipav.org/main/jpp/index.php/jpp/article/view/3738


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