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dc.contributor.author
Romanelli, Federica  
dc.contributor.author
Zenclussen, Maria Laura  
dc.contributor.author
Zenclussen, Ana Claudia  
dc.contributor.author
Meyer, Nicole  
dc.date.available
2024-02-07T15:26:48Z  
dc.date.issued
2023-11  
dc.identifier.citation
Romanelli, Federica; Zenclussen, Maria Laura; Zenclussen, Ana Claudia; Meyer, Nicole; Carbon Monoxide Exposure Does Not Improve The In Vitro Fertilization Rate of Oocytes Obtained from Heterozygous Hmox1 Knockout Mice; Bulletin Ecological Society of America; International Journal Of Fertility And Sterility; 18; 1; 11-2023; 76-80  
dc.identifier.issn
2008-076X  
dc.identifier.uri
http://hdl.handle.net/11336/226170  
dc.description.abstract
In our experimental study we explored the impact of maternal reduced heme oxygenase-1 (HO-1) gene (Hmox1) expression on the in vitro fertilization (IVF) rate through the use of heterozygous Hmox1 knockout mice models (HET/Hmox1+/-). Also, we hypothesized a beneficial role of gametes exposure during fertilization to carbon monoxide (CO), one of HO-1 by-products, that might be relevant for the improvement of IVF rates. IVF technique was performed by using oocytes obtained from wild-type (WT) or Hmox1+/- dams fertilized with WT, Hmox1+/- or Hmox1-/- mice-derived sperm. The fertilization step occurred either in a conventional incubator (37°C, 5% CO2) or in an incubator implemented with CO (500 ppm). The superovulation yield of WT and Hmox1+/- mice and the number of fertilized oocytes was assessed using an optical microscope. The dams’ Hmox1 heterozygous knockout neither impact the superovulation yield, nor did influence the fertilization success rate. Moreover, CO exposure during fertilization could not significantly improve the outcome. Our study showed that the maternal Hmox1+/- condition is not affecting the IVF rate in mice. Furthermore, we discovered that CO exposure cannot be exploited to ameliorate this critical step of the IVF protocol.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Bulletin Ecological Society of America  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc/2.5/ar/  
dc.subject
CARBON MONOXIDE  
dc.subject
GENE KNOCKOUT  
dc.subject
HO-1 PROTEIN  
dc.subject
IN VITRO FERTILIZATION  
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PREGNANCY  
dc.subject.classification
Biología Reproductiva  
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Ciencias Biológicas  
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS  
dc.title
Carbon Monoxide Exposure Does Not Improve The In Vitro Fertilization Rate of Oocytes Obtained from Heterozygous Hmox1 Knockout Mice  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2024-02-06T11:03:08Z  
dc.identifier.eissn
2008-0778  
dc.journal.volume
18  
dc.journal.number
1  
dc.journal.pagination
76-80  
dc.journal.pais
Irán  
dc.description.fil
Fil: Romanelli, Federica. Universitat Leipzig; Alemania  
dc.description.fil
Fil: Zenclussen, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; Argentina  
dc.description.fil
Fil: Zenclussen, Ana Claudia. Universitat Leipzig; Alemania  
dc.description.fil
Fil: Meyer, Nicole. Universitat Leipzig; Alemania  
dc.journal.title
International Journal Of Fertility And Sterility  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.ijfs.ir/article_705970.html  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.22074/ijfs.2023.1982726.1411