Artículo
Porphyrin-polyethylenimine conjugates as photodynamic polymers to eliminate Staphylococcus aureus and Escherichia coli
Boarini, Milena Belen
; Gsponer, Natalia Soledad
; Milanesio, María Elisa
; Durantini, Edgardo Néstor
Fecha de publicación:
14/10/2023
Editorial:
Pergamon-Elsevier Science Ltd
Revista:
European Polymer Journal
ISSN:
0014-3057
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
The increase in bacteria resistant to antibiotics in clinical use has led to the establishment of new treatments for infections. One of the proposed therapies includes photodynamic inactivation (PDI) of microorganisms. In this context, two novel polymeric conjugates, PEI-TPPF10 and PEI-TPPF20, were synthesized by the aromatic nucleophilic substitution reaction between polyethylenimine (PEI) and 5,15-di(4-N,N-dimethylaminophenyl)-10,20-di(pentafluorophenyl)porphyrin (TPPF10) or 5,10,15,20-tetrakis(pentafluorophenyl)porphyrin (TPPF20), respectively. The structures of these polymers were characterized by FTIR. UV–visible absorption and fluorescence studies indicated that the porphyrin units maintain their spectroscopic characteristics in the conjugates. The polymeric materials were able to produce singlet molecular oxygen and superoxide anion radical in solution. Furthermore, they were effective photosensitizers to photooxidize L-tryptophan and produce iodine in presence of iodide anions. The ability of these conjugates to photoinactivate bacteria was tested against Staphylococcus aureus and Escherichia coli. A reduction of 7 log (>99.9999 %) in cell survival was found after an irradiation with white light (90 mW/cm2) of 5 min (27 J/cm2) and 15 min (81 J/cm2), respectively. In these conjugates, the remaining amino groups are precursors of positive charges in the biological medium, favoring the interaction with microbial cells, which improves the photokilling activity. The results indicate that both conjugates are promising photosensitizing polymeric materials for the eradication of pathogens.
Palabras clave:
BACTERIA
,
PHOTODYNAMIC INACTIVATION
,
POLYETHYLENIMINE
,
POLYMERS
,
PORPHYRINS
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Articulos (IDAS)
Articulos de INSTITUTO PARA EL DESARROLLO AGROINDUSTRIAL Y DE LA SALUD
Articulos de INSTITUTO PARA EL DESARROLLO AGROINDUSTRIAL Y DE LA SALUD
Citación
Boarini, Milena Belen; Gsponer, Natalia Soledad; Milanesio, María Elisa; Durantini, Edgardo Néstor; Porphyrin-polyethylenimine conjugates as photodynamic polymers to eliminate Staphylococcus aureus and Escherichia coli; Pergamon-Elsevier Science Ltd; European Polymer Journal; 200; 112512; 14-10-2023; 1-10
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