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dc.contributor.author
Rey, Hebe Yolanda
dc.contributor.author
Faloci, Mirta Mabel
dc.contributor.author
Medina, Ricardo Daniel
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Dolce, Natalia Raquel
dc.contributor.author
Engelmann, Florent
dc.contributor.author
Mroginski, Luis Amado
dc.date.available
2015-10-01T18:35:25Z
dc.date.issued
2013-12
dc.identifier.citation
Rey, Hebe Yolanda; Faloci, Mirta Mabel; Medina, Ricardo Daniel; Dolce, Natalia Raquel; Engelmann, Florent; et al.; Cryopreservation of Arachis pintoi (Leguminosae) somatic embryos.; Cryo Letters; Cryo-letters; 34; 6; 12-2013; 571-582
dc.identifier.issn
0143-2044
dc.identifier.uri
http://hdl.handle.net/11336/2255
dc.description.abstract
In this study, we successfully cryopreserved cotyledonary somatic embryos of diploid and triploid Arachis pintoi cytotypes using the encapsulation-dehydration technique. The highest survival rates were obtained when somatic embryos were encapsulated in calcium alginate beads and precultured in agitated (80 rpm) liquid establishment medium (EM) with daily increasing sucrose concentration (0.50, 0.75, and 1.0 M). The encapsulated somatic embryos were then dehydrated with silica gel for 5 h to 20% moisture content (fresh weight basis) and cooled either rapidly (direct immersion in liquid nitrogen, LN) or slowly (1ºC min-1 from 25ºC to -30ºC followed by immersion in LN). Beads were kept in LN for a minimum of 1 h and then were rapidly rewarmed in a 30ºC water-bath for 2 min. Finally, encapsulated somatic embryos were post-cultured in agitated (80 rpm) liquid EM with daily decreasing sucrose concentration (0.75 and 0.5 M) and transferred to solidified EM. Using this protocol, we obtained 26% and 30% plant regeneration from cryopreserved somatic embryos of diploid and triploid cytotypes. No morphological abnormalities were observed in any of the plants regenerated from cryopreserved embryos and their genetic stability was confirmed with 10 isozyme systems and nine RAPD profiles.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Cryo Letters
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Somatic Embryo
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Cryopreservation
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Encapsulation-Dehydration
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Genetic Stability
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Ciencias de las Plantas, Botánica
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Cryopreservation of Arachis pintoi (Leguminosae) somatic embryos.
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2016-03-30 10:35:44.97925-03
dc.journal.volume
34
dc.journal.number
6
dc.journal.pagination
571-582
dc.journal.pais
Reino Unido
dc.description.fil
Fil: Rey, Hebe Yolanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); Argentina; Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina;
dc.description.fil
Fil: Faloci, Mirta Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); Argentina; Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina;
dc.description.fil
Fil: Medina, Ricardo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); Argentina; Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina;
dc.description.fil
Fil: Dolce, Natalia Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); Argentina; Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina;
dc.description.fil
Fil: Engelmann, Florent. Centre National de la Recherche Scientifique. Institut de Recherche Pour Le Developpement; Francia;
dc.description.fil
Fil: Mroginski, Luis Amado. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); Argentina; Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina;
dc.journal.title
Cryo-letters
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.ingentaconnect.com/content/cryo/cryo/2013/00000034/00000006/art00003?crawler=true
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