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Artículo

Design and characterization of chimeric Rabies-SARS-CoV-2 virus-like particles for vaccine purposes

Garay, Ernesto SergioIcon ; Fontana, Diego SebastianIcon ; Villarraza, Carlos JavierIcon ; Fuselli, AntonelaIcon ; Gugliotta, AgustinaIcon ; Antuña, Sebastián; Tardivo, Belén; Rodríguez, María CelesteIcon ; Gastaldi, VictoriaIcon ; Battagliotti, Juan ManuelIcon ; Alvarez, Diego EzequielIcon ; Castro, Eliana FlorenciaIcon ; Cassataro, JulianaIcon ; Ceaglio, Natalia AnaliaIcon ; Prieto, ClaudioIcon
Fecha de publicación: 05/2023
Editorial: Springer
Revista: Applied Microbiology and Biotechnology
ISSN: 0175-7598
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Otras Biotecnologías de la Salud

Resumen

Abstract: Due to the high number of doses required to achieve adequate coverage in the context of COVID-19 pandemics, there is a great need for novel vaccine developments. In this field, there have been research approaches that focused on the production of SARS-CoV-2 virus-like particles. These are promising vaccine candidates as their structure is similar to that of native virions but they lack the genome, constituting a biosafe alternative. In order to produce these structures using mammal cells, it has been established that all four structural proteins must be expressed. Here we report the generation and characterization of a novel chimeric virus-like particle (VLP) that can be produced by the expression of a single novel fusion protein that contains SARS-CoV-2 spike (S) ectodomain fused to rabies glycoprotein membrane anchoring region in HEK293 cells. This protein is structurally similar to native S and can autonomously bud forming enveloped VLPs that resemble native virions both in size and in morphology, displaying S ectodomain and receptor binding domain (RBD) on their surface. As a proof of concept, we analyzed the immunogenicity of this vaccine candidate in mice and confirmed the generation of anti-S, anti-RBD, and neutralizing antibodies.
Palabras clave: CHIMERIC , RABIES , RBD , SPIKE , VLP , COVID-19
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info:eu-repo/semantics/restrictedAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/224993
DOI: http://dx.doi.org/10.1007/s00253-023-12545-w
Colecciones
Articulos (IIBIO)
Articulos de INSTITUTO DE INVESTIGACIONES BIOTECNOLOGICAS
Articulos(CCT - SANTA FE)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - SANTA FE
Citación
Garay, Ernesto Sergio; Fontana, Diego Sebastian; Villarraza, Carlos Javier; Fuselli, Antonela; Gugliotta, Agustina; et al.; Design and characterization of chimeric Rabies-SARS-CoV-2 virus-like particles for vaccine purposes; Springer; Applied Microbiology and Biotechnology; 107; 11; 5-2023; 3495-3508
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