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dc.contributor.author
Morris, Ellen Ruth A.  
dc.contributor.author
Schroeder, Megan E.  
dc.contributor.author
Ferro, Pamela J.  
dc.contributor.author
Waller, Andrew Stephen  
dc.contributor.author
McGlennon, Abigail A.  
dc.contributor.author
Bustos, Carla Paola  
dc.contributor.author
Gressler, Leticia T.  
dc.contributor.author
Wu, Jing  
dc.contributor.author
Lawhon, Sara D.  
dc.contributor.author
Boyle, Ashley G.  
dc.contributor.author
Lingsweiler, Sonia  
dc.contributor.author
Paul, Narayan  
dc.contributor.author
Dimitrov, Kiril  
dc.contributor.author
Swinford, Amy K.  
dc.contributor.author
Bordin, Angela I.  
dc.contributor.author
Cohen, Noah D.  
dc.date.available
2024-01-25T14:58:35Z  
dc.date.issued
2023-09  
dc.identifier.citation
Morris, Ellen Ruth A.; Schroeder, Megan E.; Ferro, Pamela J.; Waller, Andrew Stephen; McGlennon, Abigail A.; et al.; Development of a novel real-time PCR multiplex assay for detection of Streptococcus equi subspecies equi and Streptococcus equi subspecies zooepidemicus; Elsevier Science; Veterinary Microbiology; 284; 109797; 9-2023; 1-8  
dc.identifier.issn
0378-1135  
dc.identifier.uri
http://hdl.handle.net/11336/224852  
dc.description.abstract
Strangles is a contagious bacterial disease of horses caused by Streptococcus equi subspecies equi (SEE) that occurs globally. Rapid and accurate identification of infected horses is essential for controlling strangles. Because of limitations of existing PCR assays for SEE, we sought to identify novel primers and probes that enable simultaneous detection and differentiation of infection with SEE and S. equi subsp. zooepidemicus (SEZ). Comparative genomics of U.S. strains of SEE and SEZ (n = 50 each) identified SE00768 from SEE and comB from SEZ as target genes. Primers and probes for real-time PCR (rtPCR) were designed for these genes and then aligned in silico with the genomes of strains of SEE (n = 725) and SEZ (n = 343). Additionally, the sensitivity and specificity relative to microbiologic culture were compared between 85 samples submitted to an accredited veterinary medical diagnostic laboratory. The respective primer and probe sets aligned with 99.7 % (723/725) isolates of SEE and 97.1 % (333/343) of SEZ. Of 85 diagnostic samples, 20 of 21 (95.2 %) SEE and 22 of 23 SEZ (95.6 %) culture-positive samples were positive by rtPCR for SEE and SEZ, respectively. Both SEE (n = 2) and SEZ (n = 3) were identified by rtPCR among 32 culture-negative samples. Results were rtPCR-positive for both SEE and SEZ in 21 of 44 (47.7 %) samples that were culture-positive for SEE or SEZ. The primers and probe sets reported here reliably detect SEE and SEZ from Europe and the U.S., and permit detection of concurrent infection with both subspecies.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Elsevier Science  
dc.rights
info:eu-repo/semantics/restrictedAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/  
dc.subject
COINFECTION  
dc.subject
DIAGNOSTIC LABORATORY  
dc.subject
REAL-TIME PCR  
dc.subject
STREPTOCOCCUS EQUI SUBSP. EQUI  
dc.subject
STREPTOCOCCUS EQUI SUBSP. ZOOEPIDEMICUS  
dc.subject.classification
Ciencias Veterinarias  
dc.subject.classification
Ciencias Veterinarias  
dc.subject.classification
CIENCIAS AGRÍCOLAS  
dc.title
Development of a novel real-time PCR multiplex assay for detection of Streptococcus equi subspecies equi and Streptococcus equi subspecies zooepidemicus  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2024-01-18T15:11:58Z  
dc.journal.volume
284  
dc.journal.number
109797  
dc.journal.pagination
1-8  
dc.journal.pais
Países Bajos  
dc.journal.ciudad
Amsterdam  
dc.description.fil
Fil: Morris, Ellen Ruth A.. Texas A&M University; Estados Unidos  
dc.description.fil
Fil: Schroeder, Megan E.. Texas A&M University; Estados Unidos  
dc.description.fil
Fil: Ferro, Pamela J.. Texas A&M University; Estados Unidos  
dc.description.fil
Fil: Waller, Andrew Stephen. Intervacc Ab; Suecia. Sveriges Lantbruksuniversitet (slu);  
dc.description.fil
Fil: McGlennon, Abigail A.. University of London; Reino Unido  
dc.description.fil
Fil: Bustos, Carla Paola. Universidad de Buenos Aires. Facultad de Ciencias Veterinaria. Instituto de Investigaciones En Epidemiologia Veterinaria; . Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario; Argentina  
dc.description.fil
Fil: Gressler, Leticia T.. Instituto Federal Farroupilha; Brasil  
dc.description.fil
Fil: Wu, Jing. Texas A&M University; Estados Unidos  
dc.description.fil
Fil: Lawhon, Sara D.. Texas A&M University; Estados Unidos  
dc.description.fil
Fil: Boyle, Ashley G.. University of Pennsylvania; Estados Unidos  
dc.description.fil
Fil: Lingsweiler, Sonia. Texas A&M University; Estados Unidos  
dc.description.fil
Fil: Paul, Narayan. Texas A&M University; Estados Unidos  
dc.description.fil
Fil: Dimitrov, Kiril. Texas A&M University; Estados Unidos  
dc.description.fil
Fil: Swinford, Amy K.. Texas A&M University; Estados Unidos  
dc.description.fil
Fil: Bordin, Angela I.. Texas A&M University; Estados Unidos  
dc.description.fil
Fil: Cohen, Noah D.. Texas A&M University; Estados Unidos  
dc.journal.title
Veterinary Microbiology  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.vetmic.2023.109797  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0378113523001499