Artículo
Evaluation of the susceptibility of Tritrichomonas foetus to extracts of Lantana camara (Verbenaceae) by flow cytometry
Lopez, Lucia Alejandra
; Luque, Melchor Emilio
; Rivero, María Belén
; Abdala, María Eugenia
; Carranza, Pedro Gabriel
; Luna, Bruno Elias
; Di Lullo, David
; Volta, Bibiana Julieta
; Rodríguez, Sergio Antonio
; Rivero, Fernando David
Fecha de publicación:
09/2023
Editorial:
Springer
Revista:
Veterinary Research Communications
ISSN:
0165-7380
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Bovine Trichomonosis (BT), a sexually transmitted disease endemic in countries with extensive cattle farming and natural service, is one of the most common causes of reproductive failure. 5-nitroimidazoles and their derivatives are used for its treatment, mainly metronidazole. The emergence of drug resistance mechanisms and treatment failures raise the need to investigate the effectiveness of new active compounds that contribute to parasite control. In this regard, extracts of Lantana camara (Verbenacea) have shown high biocidal potential against isolates of Trypanosoma cruzi and Leishmania braziliensis in vitro assays, although their effect on Tritrichomonas foetus has not been demonstrated yet. The available information on in vitro susceptibility of trichomonicidal drugs comes from the use of a diversity of methodologies and criteria, especially the observation of parasite motility under the optical microscope to assess their viability. Recently, in our laboratory, the use of flow cytometry has been described for the first time as a rapid and efficient method to evaluate the viability of T. foetus against metronidazole. The present study aimed to evaluate the cytostatic effect of L. camara extracts against T. foetus isolates by flow cytometry. Under aerobic conditions, IC50 values of 22.60 µg/mL were obtained on average. Under anaerobic conditions, the IC50 oscilated around 29.04 µg/mL. The results obtained allowed describing the susceptibility exhibited by these protozoa, being a valuable information for the development of potential BT treatments.
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Articulos (IMSATED)
Articulos de INSTITUTO MULTIDISCIPLINARIO DE SALUD, TECNOLOGIA Y DESARROLLO
Articulos de INSTITUTO MULTIDISCIPLINARIO DE SALUD, TECNOLOGIA Y DESARROLLO
Articulos(CCT - NOA SUR)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - NOA SUR
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - NOA SUR
Citación
Lopez, Lucia Alejandra; Luque, Melchor Emilio; Rivero, María Belén; Abdala, María Eugenia; Carranza, Pedro Gabriel; et al.; Evaluation of the susceptibility of Tritrichomonas foetus to extracts of Lantana camara (Verbenaceae) by flow cytometry; Springer; Veterinary Research Communications; 47; 3; 9-2023; 1687-1695
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