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dc.contributor.author
Felsztyna, Iván
dc.contributor.author
Perillo, Maria Angelica
dc.contributor.author
Clop, Eduardo Matias
dc.date.available
2024-01-18T14:54:06Z
dc.date.issued
2023-08
dc.identifier.citation
Felsztyna, Iván; Perillo, Maria Angelica; Clop, Eduardo Matias; Nanoarchitectonic approaches for measuring the catalytic behavior of a membrane anchored enzyme: From Langmuir-Blodgett to a novel Langmuir-Schaefer based nanofilm building device; Elsevier Science; Biochimica et Biophysica Acta - Biomembranes; 1865; 6; 8-2023; 1-25
dc.identifier.issn
0005-2736
dc.identifier.uri
http://hdl.handle.net/11336/224135
dc.description.abstract
Self-organized lipid monolayers at the air-water interface (Langmuir films, LF) are commonly used for measuring the catalytic properties of membrane-bound enzymes. This methodology allows to provide a consistent flat topography molecular density, packing defects and thickness. The aim of the present work was to show the methodological advantages of using the horizontal transfer method (Langmuir-Schaefer) with respect to the vertical transfer method (Langmuir-Blodgett) when mounting a device to measure catalytic activity of membrane enzymes. Based on the results obtained we can conclude that it is possible to prepare stable Langmuir-Blodgett (LB) and Langmuir-Schaefer (LS) films from Bovine Erythrocyte Membranes (BEM) preserving the catalytic activity of its native Acetylcholinesterase (BEA). In comparison, the LS films showed Vmax values more similar to the enzyme present in the vesicles of natural membranes. In addition, it was much easier to produce large amounts of transferred areas with the horizontal transfer methodology. It was possible to decrease the time required to mount an assay with numerous activity points, such as building activity curves as a function of substrate concentration. The present results show that LSBEM provides a proof of concept for the development of biosensors based on transferred purified membrane for the screening of new products acting on an enzyme embedded on its natural milieu. In the case of BEA, the application of these enzymatic sensors could have medical interest, providing drug screening tools for the treatment of Alzheimer's disease.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Science
dc.rights
info:eu-repo/semantics/embargoedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
BIOSENSOR PROOF OF PRINCIPLE
dc.subject
ERYTHROCYTE ACETYLCHOLINESTERASE
dc.subject
ERYTHROCYTE GHOST MEMBRANES
dc.subject
LANGMUIR-BLODGETT FILMS
dc.subject
LANGMUIR-SCHAEFER FILMS
dc.subject.classification
Biofísica
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Nanoarchitectonic approaches for measuring the catalytic behavior of a membrane anchored enzyme: From Langmuir-Blodgett to a novel Langmuir-Schaefer based nanofilm building device
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2024-01-03T12:33:26Z
dc.journal.volume
1865
dc.journal.number
6
dc.journal.pagination
1-25
dc.journal.pais
Países Bajos
dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Felsztyna, Iván. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
dc.description.fil
Fil: Perillo, Maria Angelica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
dc.description.fil
Fil: Clop, Eduardo Matias. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
dc.journal.title
Biochimica et Biophysica Acta - Biomembranes
dc.rights.embargoDate
2024-02-18
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0005273623000597
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.bbamem.2023.184177
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