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dc.contributor.author
Caggiano, Nicolás
dc.contributor.author
Crespi, Elisa Silvia
dc.contributor.author
Fiorini, Gonzalo
dc.contributor.author
de Simone, Emilio Adrian
dc.date.available
2024-01-17T12:40:46Z
dc.date.issued
2022-03
dc.identifier.citation
Caggiano, Nicolás; Crespi, Elisa Silvia; Fiorini, Gonzalo; de Simone, Emilio Adrian; Levels of milk glycosaminoglycans in Holstein cows with clinical and subclinical mastitis; Scientific Technical Research Council Turkey-tubitak; Turkish Journal Of Veterinary And Animal Sciences; 46; 2; 3-2022; 171-179
dc.identifier.issn
1300-0128
dc.identifier.uri
http://hdl.handle.net/11336/223905
dc.description.abstract
Mastitis is an inflammation of the mammary gland whose diagnosis is based on clinical criteria and the determination of inflammatory markers. The disease is mainly caused by bacteria causing intra-mammary infections. The etiological agent/s are detected by milk culture or by molecular biology approaches. However, these methodologies are unpractical for on-farm diagnosis, where rapid decisions must be made when infected animals are detected. For this reason, the levels of glycosaminoglycans (GAGs) in milk were evaluated as a tool for the diagnosis of mastitis. GAGs levels were determined by the dimethylmethylene blue assay (DMMB) method in milk from healthy animals and in milk from animals with mastitis caused by different etiological agents. Low levels of GAGs (1.93 ± 0.40 mg) were found in milk samples obtained from healthy animals (H), while intermediate (2.4 ± 0.61 mg) and high levels (2.87 ± 0.7 mg) were found in milk obtained from cows with subclinical (SM) and clinical mastitis (CM), respectively. Statistically significant differences were found between the SM and H groups (p < 0.001) and between CM and the other groups (p < 0.0001). The highest levels of GAGs were detected in the CM group, in which coagulase negative Staphylococcus (CNS) and Streptococcus spp. were isolated. Animals infected by S. aureus had the highest levels of GAGs in the SM group. The sensitivity (Se) and specificity (Sp) for the assessment of GAGs levels for CM diagnosis were 89.7 % and 81.6 %, respectively. For the diagnosis of SM, Se and Sp were 73.2 % and 95 %, respectively. The area under the curve for the diagnosis of CM was 0.902 (p < 0.0001), while that for SM was 0.833 (p < 0.0001). Results suggest that the measurement of GAGs levels in milk can be used as an indicator of udder health in dairy cattle, since increased levels of GAGs were associated with mastitis.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Scientific Technical Research Council Turkey-tubitak
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
BOVINE MASTITIS
dc.subject
GLYCOSAMINOGLYCANS
dc.subject
MILK
dc.subject.classification
Producción Animal y Lechería
dc.subject.classification
Producción Animal y Lechería
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CIENCIAS AGRÍCOLAS
dc.title
Levels of milk glycosaminoglycans in Holstein cows with clinical and subclinical mastitis
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2024-01-11T10:01:54Z
dc.journal.volume
46
dc.journal.number
2
dc.journal.pagination
171-179
dc.journal.pais
Turquía
dc.description.fil
Fil: Caggiano, Nicolás. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Fisiología Animal; Argentina
dc.description.fil
Fil: Crespi, Elisa Silvia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Fiorini, Gonzalo. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Fisiología Animal; Argentina
dc.description.fil
Fil: de Simone, Emilio Adrian. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Fisiología Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.journal.title
Turkish Journal Of Veterinary And Animal Sciences
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.55730/1300-0128.4164
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