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dc.contributor.author
Carriquiriborde, Pedro
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dc.contributor.author
Fernandino, Juan Ignacio
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dc.contributor.author
López, Carina G.
dc.contributor.author
de San Benito, Eduardo José
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dc.contributor.author
Gutierrez Villagomez, Juan Manuel
dc.contributor.author
Cristos, Diego Sebastián
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dc.contributor.author
Trudeau, Vance L.
dc.contributor.author
Somoza, Gustavo Manuel
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dc.date.available
2024-01-10T15:33:11Z
dc.date.issued
2023-01
dc.identifier.citation
Carriquiriborde, Pedro; Fernandino, Juan Ignacio; López, Carina G.; de San Benito, Eduardo José; Gutierrez Villagomez, Juan Manuel; et al.; Atrazine alters early sexual development of the South American silverside, Odontesthes bonariensis; Elsevier Science; Aquatic Toxicology; 254; 1-2023; 1-9
dc.identifier.issn
0166-445X
dc.identifier.uri
http://hdl.handle.net/11336/223255
dc.description.abstract
Atrazine (ATZ) is a frequent contaminant in freshwater ecosystems within agricultural regions. The capacity of this herbicide to interfere with the vertebrate endocrine system is broadly recognized, but the mechanisms and responses usually differ among species. In this study, ATZ effects on hypothalamus-pituitary-gonadal (HPG) axis key genes expression and early gonadal development were evaluated in Odontesthes bonariensis larvae waterborne exposed during the gonadal differentiation period. Fish were treated to 0, 0.7, 7.0, and 70 µg ATZ/L at 25 °C from the 2nd to 6th week after hatching (wah), and a group was kept in clean water until the 12th wah. Parallelly, a group was submitted to 0.05 µg/L of ethinylestradiol (EE2) as a positive estrogenic control. From each treatment, eight larvae were sampled at 6 wah for gene expression analysis and twelve larvae at 12 wah for phenotypic sex histological determination. The expression of gnrh1, lhb, fshb, and cyp19a1b was assessed in the head, and the ones of amha, 11βhsd2, and cyp19a1a in the trunk. Fish growth was significantly higher in fish exposed to 7 and 70 µg ATZ/L in the 6 wah, but the effect vanished at the 12 wah. The expression of lhb was upregulated in both sex larvae exposed from 7 µg ATZ/L. However, a dimorphic effect was induced on cyp19a1a expression at 70 µg ATZ/L, up or downregulating mRNA transcription in males and females, respectively. Delayed ovarian development and increased number of testicular germ cells were histologically observed from 7 to 70 µg ATZ/L, respectively, and a sex inversion (genotypic male to phenotypic female) was found in one larva at 70 µg ATZ/L. The lhb expression was also upregulated by EE2, but the cyp19a1a expression was not affected, and a complete male-to-female reversal was induced. Further, EE2 upregulated gnrh1 in females and cyp19a1b in both sexes, but it did not alter any assessed gene in the trunk. In conclusion, ATZ disrupted HPG axis physiology and normal gonadal development in O. bonariensis larvae at environmentally relevant concentrations. The responses to ATZ only partially overlapped and were less active when compared to the model estrogenic compound EE2.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Science
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dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
ENDOCRINE DISRUPTION
dc.subject
FISH
dc.subject
GENE EXPRESSION
dc.subject
GONADAL DIFFERENTIATION
dc.subject
PESTICIDES
dc.subject.classification
Zoología, Ornitología, Entomología, Etología
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dc.subject.classification
Ciencias Biológicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
Atrazine alters early sexual development of the South American silverside, Odontesthes bonariensis
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2024-01-09T15:04:58Z
dc.journal.volume
254
dc.journal.pagination
1-9
dc.journal.pais
Países Bajos
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dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Carriquiriborde, Pedro. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigaciones del Medio Ambiente - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigaciones del Medio Ambiente; Argentina
dc.description.fil
Fil: Fernandino, Juan Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina
dc.description.fil
Fil: López, Carina G.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina
dc.description.fil
Fil: de San Benito, Eduardo José. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigaciones del Medio Ambiente - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigaciones del Medio Ambiente; Argentina
dc.description.fil
Fil: Gutierrez Villagomez, Juan Manuel. University of Ottawa; Canadá
dc.description.fil
Fil: Cristos, Diego Sebastián. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Agroindustria; Argentina
dc.description.fil
Fil: Trudeau, Vance L.. University of Ottawa; Canadá
dc.description.fil
Fil: Somoza, Gustavo Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina
dc.journal.title
Aquatic Toxicology
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.aquatox.2022.106366
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