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dc.contributor.author
Figueroa, Ana Cristina  
dc.contributor.author
Díaz, María Soledad  
dc.contributor.author
Alasino, Roxana Valeria  
dc.contributor.author
Yang, Pablo  
dc.contributor.author
Soria, Néstor Walter  
dc.contributor.author
Beltramo, Dante Miguel  
dc.date.available
2024-01-05T15:17:09Z  
dc.date.issued
2022-10  
dc.identifier.citation
Figueroa, Ana Cristina; Díaz, María Soledad; Alasino, Roxana Valeria; Yang, Pablo; Soria, Néstor Walter; et al.; Expression and localization of tubulin isotypes and its mRNAs during Thecaphora frezii developments; Wiley Blackwell Publishing, Inc; Fems Microbiology Ecology; 98; 11; 10-2022  
dc.identifier.issn
0168-6496  
dc.identifier.uri
http://hdl.handle.net/11336/222631  
dc.description.abstract
Thecaphora frezii is a phytopathogenic fungus that infects Arachys hypogaea L. and produces peanut smut. It has three ontological stages teliospores, basidiospores, and hyphae. Microtubules are cellular structures that participate in various important cellular processes. In this work, we analyzed the presence and location of α-tubulin isotypes and enzymes that participate in tyrosination-detyrosination in the three stages of T. frezii. Although both tyrosinated and detyrosinated tubulin seem to be associated with a membrane fraction component that gives it a similar behavior to integral proteins, in the soluble cytosolic fraction, only detyrosinated tubulin was detected, not tyrosinated tubulin. The presence of α-tubulin was not detected using the monoclonal antibody DM1A as neither acetylated tubulin. The RNA-Seq analysis showed the presence of α, β, and γ-tubulins and the genes that codes for tyrosine-tubulin ligase and cytosolic carboxypeptidase 1, enzymes that are involved in post-translational modification processes. These sequences showed a high percentage of identity and homology with Ustilago maydis, Thecaphora thlaspeos, and Anthracocystis flocculosa. This is the first report for tubulins subpopulations and the cellular distribution in T. frezii, which together with the data obtained by RNA-Seq contribute to the knowledge of the pathogen, which will allow the development of control strategies  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Wiley Blackwell Publishing, Inc  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
CYTOSQUELETON  
dc.subject
DETYROSINATED TUBULIN  
dc.subject
IMMUNOSTAINING  
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MRNA SEQUENCE  
dc.subject
PEANUT SMUT  
dc.subject
TYROSINATED TUBULIN  
dc.subject.classification
Biología Celular, Microbiología  
dc.subject.classification
Ciencias Biológicas  
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CIENCIAS NATURALES Y EXACTAS  
dc.title
Expression and localization of tubulin isotypes and its mRNAs during Thecaphora frezii developments  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2024-01-04T10:55:16Z  
dc.journal.volume
98  
dc.journal.number
11  
dc.journal.pais
Reino Unido  
dc.journal.ciudad
Londres  
dc.description.fil
Fil: Figueroa, Ana Cristina. Provincia de Córdoba. Ministerio de Ciencia y Técnica. Centro de Excelencia en Productos y Procesos de Córdoba; Argentina  
dc.description.fil
Fil: Díaz, María Soledad. Provincia de Córdoba. Ministerio de Ciencia y Técnica. Centro de Excelencia en Productos y Procesos de Córdoba; Argentina  
dc.description.fil
Fil: Alasino, Roxana Valeria. Provincia de Córdoba. Ministerio de Ciencia y Técnica. Centro de Excelencia en Productos y Procesos de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina  
dc.description.fil
Fil: Yang, Pablo. Universidad Católica de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina  
dc.description.fil
Fil: Soria, Néstor Walter. Universidad Católica de Córdoba; Argentina  
dc.description.fil
Fil: Beltramo, Dante Miguel. Universidad Católica de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Provincia de Córdoba. Ministerio de Ciencia y Técnica. Centro de Excelencia en Productos y Procesos de Córdoba; Argentina  
dc.journal.title
Fems Microbiology Ecology  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1093/femsec/fiac121  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/femsec/article/98/11/fiac121/6753202