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dc.contributor.author
Figueroa, Ana Cristina
dc.contributor.author
Díaz, María Soledad
dc.contributor.author
Alasino, Roxana Valeria
dc.contributor.author
Yang, Pablo
dc.contributor.author
Soria, Néstor Walter
dc.contributor.author
Beltramo, Dante Miguel
dc.date.available
2024-01-05T15:17:09Z
dc.date.issued
2022-10
dc.identifier.citation
Figueroa, Ana Cristina; Díaz, María Soledad; Alasino, Roxana Valeria; Yang, Pablo; Soria, Néstor Walter; et al.; Expression and localization of tubulin isotypes and its mRNAs during Thecaphora frezii developments; Wiley Blackwell Publishing, Inc; Fems Microbiology Ecology; 98; 11; 10-2022
dc.identifier.issn
0168-6496
dc.identifier.uri
http://hdl.handle.net/11336/222631
dc.description.abstract
Thecaphora frezii is a phytopathogenic fungus that infects Arachys hypogaea L. and produces peanut smut. It has three ontological stages teliospores, basidiospores, and hyphae. Microtubules are cellular structures that participate in various important cellular processes. In this work, we analyzed the presence and location of α-tubulin isotypes and enzymes that participate in tyrosination-detyrosination in the three stages of T. frezii. Although both tyrosinated and detyrosinated tubulin seem to be associated with a membrane fraction component that gives it a similar behavior to integral proteins, in the soluble cytosolic fraction, only detyrosinated tubulin was detected, not tyrosinated tubulin. The presence of α-tubulin was not detected using the monoclonal antibody DM1A as neither acetylated tubulin. The RNA-Seq analysis showed the presence of α, β, and γ-tubulins and the genes that codes for tyrosine-tubulin ligase and cytosolic carboxypeptidase 1, enzymes that are involved in post-translational modification processes. These sequences showed a high percentage of identity and homology with Ustilago maydis, Thecaphora thlaspeos, and Anthracocystis flocculosa. This is the first report for tubulins subpopulations and the cellular distribution in T. frezii, which together with the data obtained by RNA-Seq contribute to the knowledge of the pathogen, which will allow the development of control strategies
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Wiley Blackwell Publishing, Inc
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
CYTOSQUELETON
dc.subject
DETYROSINATED TUBULIN
dc.subject
IMMUNOSTAINING
dc.subject
MRNA SEQUENCE
dc.subject
PEANUT SMUT
dc.subject
TYROSINATED TUBULIN
dc.subject.classification
Biología Celular, Microbiología
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Expression and localization of tubulin isotypes and its mRNAs during Thecaphora frezii developments
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2024-01-04T10:55:16Z
dc.journal.volume
98
dc.journal.number
11
dc.journal.pais
Reino Unido
dc.journal.ciudad
Londres
dc.description.fil
Fil: Figueroa, Ana Cristina. Provincia de Córdoba. Ministerio de Ciencia y Técnica. Centro de Excelencia en Productos y Procesos de Córdoba; Argentina
dc.description.fil
Fil: Díaz, María Soledad. Provincia de Córdoba. Ministerio de Ciencia y Técnica. Centro de Excelencia en Productos y Procesos de Córdoba; Argentina
dc.description.fil
Fil: Alasino, Roxana Valeria. Provincia de Córdoba. Ministerio de Ciencia y Técnica. Centro de Excelencia en Productos y Procesos de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
dc.description.fil
Fil: Yang, Pablo. Universidad Católica de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
dc.description.fil
Fil: Soria, Néstor Walter. Universidad Católica de Córdoba; Argentina
dc.description.fil
Fil: Beltramo, Dante Miguel. Universidad Católica de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Provincia de Córdoba. Ministerio de Ciencia y Técnica. Centro de Excelencia en Productos y Procesos de Córdoba; Argentina
dc.journal.title
Fems Microbiology Ecology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1093/femsec/fiac121
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/femsec/article/98/11/fiac121/6753202
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