Mostrar el registro sencillo del ítem
dc.contributor.author
Mangano, Silvina
dc.contributor.author
Muñoz, Alfonso
dc.contributor.author
Fernández Calvino, Lourdes
dc.contributor.author
Castellano, M. Mar
dc.date.available
2024-01-02T11:05:16Z
dc.date.issued
2023-05
dc.identifier.citation
Mangano, Silvina; Muñoz, Alfonso; Fernández Calvino, Lourdes; Castellano, M. Mar; HOP co-chaperones contribute to GA signaling by promoting the accumulation of the F-box protein SNE in Arabidopsis; Cell Press; Plant Communications; 4; 3; 5-2023; 1-14
dc.identifier.issn
2590-3462
dc.identifier.uri
http://hdl.handle.net/11336/221916
dc.description.abstract
Gibberellins (GAs) play important roles in multiple developmental processes and in plant response to the environment. Within the GA pathway, a central regulatory step relies on GA-dependent degradation of the DELLA transcriptional regulators. Nevertheless, the relevance of the stability of other key proteins in this pathway, such as SLY1 and SNE (the F-box proteins involved in DELLA degradation), remains unknown. Here, we take advantage of mutants in the HSP70-HSP90 organizing protein (HOP) co-chaperones and reveal that these proteins contribute to the accumulation of SNE in Arabidopsis. Indeed, HOP proteins, along with HSP90 and HSP70, interact in vivo with SNE, and SNE accumulation is significantly reduced in the hop mutants. Concomitantly, greater accumulation of the DELLA protein RGA is observed in these plants. In agreement with these molecular phenotypes, hop mutants show a hypersensitive response to the GA inhibitor paclobutrazol and display a partial response to the ectopic addition of GA when GA-regulated processes are assayed. These mutants also display different phenotypes associated with alterations in the GA pathway, such as reduced germination rate, delayed bolting, and reduced hypocotyl elongation in response to warm temperatures. Remarkably, ectopic overexpression of SNE reverts the delay in germination and the thermally dependent hypocotyl elongation defect of the hop1 hop2 hop3 mutant, revealing that SNE accumulation is the key aspect of the hop mutant phenotypes. Together, these data reveal a pivotal role for HOP in SNE accumulation and GA signaling.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Cell Press
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.subject
HSP70-HSP90 ORGANIZING PROTEIN
dc.subject
PROTEIN FOLDING
dc.subject
PROTEIN STABILITY
dc.subject
RGA
dc.subject
SEED GERMINATION
dc.subject
THERMOMORPHOGENESIS
dc.subject.classification
Bioquímica y Biología Molecular
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
HOP co-chaperones contribute to GA signaling by promoting the accumulation of the F-box protein SNE in Arabidopsis
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2023-12-27T17:45:31Z
dc.journal.volume
4
dc.journal.number
3
dc.journal.pagination
1-14
dc.journal.pais
Países Bajos
dc.description.fil
Fil: Mangano, Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina. Universidad Politécnica de Madrid; España
dc.description.fil
Fil: Muñoz, Alfonso. Universidad de Córdoba; España. Universidad Politécnica de Madrid; España
dc.description.fil
Fil: Fernández Calvino, Lourdes. Universidad Politécnica de Madrid; España. Consejo Superior de Investigaciones Científicas; España
dc.description.fil
Fil: Castellano, M. Mar. Consejo Superior de Investigaciones Científicas; España. Universidad Politécnica de Madrid; España
dc.journal.title
Plant Communications
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S2590346223000032
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.xplc.2023.100517
Archivos asociados