Do environmentally relevant concentrations of the neonicotinoid insecticide imidacloprid induce DNA damage and oxidative stress on Cnesterodon decemmaculatus (Jenyns, 1842)?

Abstract

Lethal and sublethal effects of imidacloprid (IMI) were assessed on Cnesterodon decemmaculatus (Pisces: Poeciliidae) by acute exposure to environmentally relevant concentrations of the commercial formulation Punto 35® (Gleba S.A.) under laboratory conditions. Specimens were exposed for 96 h to 1, 10, 20, 25, 35, 75, 100, 125, 150 and 175 mg IMI L-1 from which an LC50 96 h value of 35.59 mg IMI L-1 was calculated. Moreover, sublethal concentrations 0.175, 0.35 and 1 mg IMI L-1 for 96 h were employed for the evaluation of the comet assay and the variation of activities of catalase (CAT) and glutathione content (GSH). Result demonstrated an increased genetic damage index and activity of CAT was observed. Conversely, no significant variation was observed in GSH activity. Total protein content decreased in treated organisms. These results represent the first report of acute effects induced by IMI on C. decemmaculatus exposed under laboratory conditions.