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dc.contributor.author
Walker, Angela K.
dc.contributor.author
Park, Won Mee
dc.contributor.author
Chuang, Jen Chieh
dc.contributor.author
Perello, Mario
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dc.contributor.author
Sakata, Ichiro
dc.contributor.author
Osborne Lawrence, Sherri
dc.contributor.author
Zigman, Jeffrey
dc.date.available
2017-08-11T15:25:39Z
dc.date.issued
2013-03
dc.identifier.citation
Walker, Angela K.; Park, Won Mee; Chuang, Jen Chieh; Perello, Mario; Sakata, Ichiro; et al.; Characterization of gastric and neuronal histaminergic populations using a transgenic mouse model; Public Library Science; Plos One; 8; 3; 3-2013; 1-12; e60276
dc.identifier.issn
1932-6203
dc.identifier.uri
http://hdl.handle.net/11336/22168
dc.description.abstract
Histamine is a potent biogenic amine that mediates numerous physiological processes throughout the body, including digestion, sleep, and immunity. It is synthesized by gastric enterochromaffin-like cells, a specific set of hypothalamic neurons, as well as a subset of white blood cells, including mast cells. Much remains to be learned about these varied histamine-producing cell populations. Here, we report the validation of a transgenic mouse line in which Cre recombinase expression has been targeted to cells expressing histidine decarboxylase (HDC), which catalyzes the rate-limiting step in the synthesis of histamine. This was achieved by crossing the HDC-Cre mouse line with Rosa26-tdTomato reporter mice, thus resulting in the expression of the fluorescent Tomato (Tmt) signal in cells containing Cre recombinase activity. As expected, the Tmt signal co-localized with HDC-immunoreactivity within the gastric mucosa and gastric submucosa and also within the tuberomamillary nucleus of the brain. HDC expression within Tmt-positive gastric cells was further confirmed by quantitative PCR analysis of mRNA isolated from highly purified populations of Tmt-positive cells obtained by fluorescent activated cell sorting (FACS). HDC expression within these FACS-separated cells was found to coincide with other markers of both ECL cells and mast cells. Gastrin expression was co-localized with HDC expression in a subset of histaminergic gastric mucosal cells. We suggest that these transgenic mice will facilitate future studies aimed at investigating the function of histamine-producing cells.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Public Library Science
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Transgenic Mice
dc.subject
Histidine Decarboxylase
dc.subject.classification
Bioquímica y Biología Molecular
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dc.subject.classification
Medicina Básica
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dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
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dc.title
Characterization of gastric and neuronal histaminergic populations using a transgenic mouse model
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2015-05-20T18:18:08Z
dc.journal.volume
8
dc.journal.number
3
dc.journal.pagination
1-12; e60276
dc.journal.pais
Estados Unidos
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dc.journal.ciudad
San Francisco
dc.description.fil
Fil: Walker, Angela K.. University of Texas; Estados Unidos
dc.description.fil
Fil: Park, Won Mee. University of Texas; Estados Unidos
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Fil: Chuang, Jen Chieh. University of Texas; Estados Unidos
dc.description.fil
Fil: Perello, Mario. University of Texas. Southwestern Medical Center; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Sakata, Ichiro. University of Texas; Estados Unidos
dc.description.fil
Fil: Osborne Lawrence, Sherri. University of Texas; Estados Unidos
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Fil: Zigman, Jeffrey. University of Texas; Estados Unidos
dc.journal.title
Plos One
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1371/journal.pone.0060276
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0060276
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