Artículo
Comprehensive characterization of Cysteine-rich protein-coding genes of Giardia lamblia and their role during antigenic variation
Rodríguez Walker, Macarena
; Molina, Cecilia Rita
; Luján, Lucas Agustín
; Saura, Alicia
; Jerlström Hultqvist, Jon; Svärd, Staffan G.; Fernandez, Elmer Andres
; Lujan, Hugo Daniel
Fecha de publicación:
08/2022
Editorial:
Academic Press Inc Elsevier Science
Revista:
Genomics
ISSN:
0888-7543
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Giardia lamblia encodes several families of cysteine-rich proteins, including the Variant-specific Surface Proteins (VSPs) involved in the process of antigenic variation. Their characteristics, definition and relationships are still controversial. An exhaustive analysis of the Cys-rich families including organization, features, evolution and levels of expression was performed, by combining pattern searches and predictions with massive sequencing techniques. Thus, a new classification for Cys-rich proteins, genes and pseudogenes that better describes their involvement in Giardia's biology is presented. Moreover, three novel characteristics exclusive to the VSP genes, comprising an Initiator element/Kozak-like sequence, an extended polyadenylation signal and a unique pattern of mutually exclusive transcript accumulation are presented, as well as the finding that High Cysteine Membrane Proteins, upregulated under stress, may protect the parasite during VSP switching. These results allow better interpretation of previous reports providing the basis for further studies of the biology of this early-branching eukaryote.
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Articulos(CIDIE)
Articulos de CENTRO DE INV. Y DESARROLLO EN INMUNOLOGIA Y ENFERMEDADES INFECCIOSAS
Articulos de CENTRO DE INV. Y DESARROLLO EN INMUNOLOGIA Y ENFERMEDADES INFECCIOSAS
Citación
Rodríguez Walker, Macarena; Molina, Cecilia Rita; Luján, Lucas Agustín; Saura, Alicia; Jerlström Hultqvist, Jon; et al.; Comprehensive characterization of Cysteine-rich protein-coding genes of Giardia lamblia and their role during antigenic variation; Academic Press Inc Elsevier Science; Genomics; 114; 5; 8-2022; 1-15
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