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dc.contributor.author
Baptista, Maurício S.  
dc.contributor.author
Cadet, Jean  
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Greer, Alexander  
dc.contributor.author
Thomas, Andrés Héctor  
dc.date.available
2023-12-22T11:06:12Z  
dc.date.issued
2023-03  
dc.identifier.citation
Baptista, Maurício S.; Cadet, Jean; Greer, Alexander; Thomas, Andrés Héctor; Practical Aspects in the Study of Biological Photosensitization Including Reaction Mechanisms and Product Analyses: A Do's and Don'ts Guide; Wiley Blackwell Publishing, Inc; Photochemistry and Photobiology; 99; 2; 3-2023; 313-334  
dc.identifier.issn
0031-8655  
dc.identifier.uri
http://hdl.handle.net/11336/221227  
dc.description.abstract
The interaction of light with natural matter leads to a plethora of photosensitized reactions. These reactions cause the degradation of biomolecules, such as DNA, lipids, proteins, being therefore detrimental to the living organisms, or they can also be beneficial by allowing the treatment of several diseases by photomedicine. Based on the molecular mechanistic understanding of the photosensitization reactions, we propose to classify them in four processes: oxygen-dependent (type I and type II processes) and oxygen-independent [triplet-triplet energy transfer (TTET) and photoadduct formation]. In here, these processes are discussed by considering a wide variety of approaches including time-resolved and steady-state techniques, together with solvent, quencher, and scavenger effects. The main aim of this survey is to provide a description of general techniques and approaches that can be used to investigate photosensitization reactions of biomolecules together with basic recommendations on good practices. Illustration of the suitability of these approaches is provided by the measurement of key biomarkers of singlet oxygen and one-electron oxidation reactions in both isolated and cellular DNA. Our work is an educational review that is mostly addressed to students and beginners.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Wiley Blackwell Publishing, Inc  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
mechanisms  
dc.subject
photosensitization  
dc.subject.classification
Bioquímica y Biología Molecular  
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Ciencias Biológicas  
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CIENCIAS NATURALES Y EXACTAS  
dc.title
Practical Aspects in the Study of Biological Photosensitization Including Reaction Mechanisms and Product Analyses: A Do's and Don'ts Guide  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2023-12-19T12:34:26Z  
dc.journal.volume
99  
dc.journal.number
2  
dc.journal.pagination
313-334  
dc.journal.pais
Reino Unido  
dc.journal.ciudad
Londres  
dc.description.fil
Fil: Baptista, Maurício S.. Universidade de Sao Paulo; Brasil  
dc.description.fil
Fil: Cadet, Jean. University of Sherbrooke; Canadá  
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Fil: Greer, Alexander. City University of New York; Estados Unidos  
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Fil: Thomas, Andrés Héctor. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina  
dc.journal.title
Photochemistry and Photobiology  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1111/php.13774