Determinacion de cromosomas holocineticos a través de la secuencia CID (cenp) en Dermaptera y Neuroptera (Insecta)

Abstract

During cell division, the centromere is an indispensable chromosomal region to ensure the segregation of geneticsinformation. In most eukaryotes, the identity of the centromere is determined by the presence of the CENP protein(CID in Diptera). Some groups of animals have chromosomes that lack localized centromeres (holocentric); a clearexample of this is Dermaptera, although the holocentricity of their chromosomes is questioned since structuressimilar to centromeres were observed in them. It is also believed that neuroptera carries chromosomes with localizedcentromeres (monocentric), but recent studies have questioned this assertion since Chrysoperla presentschromosomes lacking centromeres. The aim of this work was to determine the holocentricity of chromosomes ofDoru linerae, D. luteipes (Dermaptera) and Chrysoperla externa (Neuroptera) by amplifying the CENP gen. Usingthe Quiagen extraction kit, total DNA was obtained fromf Droshophila melanogaster, Zaprionus indianus gupta(Diptera) and Astylus atromaculatus (Coleoptera) species with monocentric chromosomes and the target species. Theprimers used were: Fw-5´GAGAACGGAGCTTGGGTT3´; Rv5´CGTCGGCGAACAACTCAAG3´. PCRamplification was performed with conventional protocols. The final product was identified in 1% agarose gel. ThePCR reaction was positive for Diptera and Coleoptera. The lack of amplicons of CENP gen in Doru and Chrysoperlais another fact that demonstrates the holocentricity of their chromosomes.