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dc.contributor.author
Balatti, Galo Ezequiel
dc.contributor.author
Martini, María Florencia
dc.contributor.author
Pickholz, Mónica Andrea
dc.date.available
2023-12-12T13:46:37Z
dc.date.issued
2023-02
dc.identifier.citation
Balatti, Galo Ezequiel; Martini, María Florencia; Pickholz, Mónica Andrea; Transient Aurein 1.2 pores in prokaryotic model bilayers explored by coarse-grain molecular dynamics simulations: as glycolipid content increases, pore lifetime decreases; Cold Spring Harbor Laboratory Press; bioRxiv; 2023; 2-2023; 1-22
dc.identifier.issn
2692-8205
dc.identifier.uri
http://hdl.handle.net/11336/219985
dc.description.abstract
The Aurein 1.2 is an anuran antimicrobial peptide (AMP) with a proven lytical activity against bacterial membranes. Previously, we found a differential action of aurein by both experimental and computational methods. This differential action was over membranes of two related probiotic strains, where the main differences between membranes were the number of glycolipids on lipid composition. In the present work, we focused on the differential behavior of the aurein 1.2 at its interaction with bacterium model membranes with different amounts of glycolipids in their composition. We carried out extensive molecular dynamics (MD) simulations by using the coarse-grain force field MARTINI and raising up differential mixtures of phosphatidylglycerol (PG), phosphatidylethanolamine (PE) and monogalactosylglycerol (MG). We found a correlation between the presence of MG in PG/PE mixtures and the difficulty of aurein to stabilize pore structures, suggesting an AMP resistance factor encoded in the lipid composition of the membrane. Through this study, we hope to shed light on a possible resistance mechanism to AMPs related with the glycolipid content of diverse types of bacterial membranes.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Cold Spring Harbor Laboratory Press
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by/2.5/ar/
dc.subject
ANTIMICROBIAL PEPTIDES
dc.subject
MOLECULAR DYNAMICS
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ANTIMICROBIAL RESISTANCE
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Biofísica
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
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Biología Celular, Microbiología
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
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Medicina Química
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Medicina Básica
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CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
Transient Aurein 1.2 pores in prokaryotic model bilayers explored by coarse-grain molecular dynamics simulations: as glycolipid content increases, pore lifetime decreases
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2023-12-07T17:51:34Z
dc.journal.volume
2023
dc.journal.pagination
1-22
dc.journal.pais
Estados Unidos
dc.description.fil
Fil: Balatti, Galo Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; Argentina
dc.description.fil
Fil: Martini, María Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; Argentina
dc.description.fil
Fil: Pickholz, Mónica Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; Argentina
dc.journal.title
bioRxiv
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.biorxiv.org/content/10.1101/2023.01.24.525384v1
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://doi.org/10.1101/2023.01.24.525384
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