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Artículo

Electrochemical Fine-Tuning of the Chemoresponsiveness of Langmuir-Blodgett Graphene Oxide Films

Devida, Juan MarceloIcon ; Herrera, Facundo CarlosIcon ; Daza Millone, Maria AntonietaIcon ; Requejo, Felix GregorioIcon ; Pallarola, Diego AndresIcon
Fecha de publicación: 07/2023
Editorial: American Chemical Society
Revista: ACS Omega
ISSN: 2470-1343
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Nano-materiales

Resumen

Graphene oxide has been widely deployed in electrical sensors for monitoring physical, chemical, and biological processes. The presence of abundant oxygen functional groups makes it an ideal substrate for integrating biological functional units to assemblies. However, the introduction of this type of defects on the surface of graphene has a deleterious effect on its electrical properties. Therefore, adjusting the surface chemistry of graphene oxide is of utmost relevance for addressing the immobilization of biomolecules, while preserving its electrochemical integrity. Herein, we describe the direct immobilization of glucose oxidase onto graphene oxide-based electrodes prepared by Langmuir-Blodgett assembly. Electrochemical reduction of graphene oxide allowed to control its surface chemistry and, by this, regulate the nature and density of binding sites for the enzyme and the overall responsiveness of the Langmuir-Blodgett biofilm. X-ray photoelectron spectroscopy, surface plasmon resonance, and electrochemical measurements were used to characterize the compositional and functional features of these biointerfaces. Covalent binding between amine groups on glucose oxidase and epoxy and carbonyl groups on the surface of graphene oxide was successfully used to build up stable and active enzymatic assemblies. This approach constitutes a simple, quick, and efficient route to locally address functional proteins at interfaces without the need for additives or complex modifiers to direct the adsorption process.
Palabras clave: biosensor , graphene oxide
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Atribución-NoComercial-SinDerivadas 2.5 Argentina (CC BY-NC-ND 2.5 AR)
Identificadores
URI: http://hdl.handle.net/11336/219929
URL: https://pubs.acs.org/doi/10.1021/acsomega.3c03220
DOI: http://dx.doi.org/10.1021/acsomega.3c03220
Colecciones
Articulos(INIFTA)
Articulos de INST.DE INV.FISICOQUIMICAS TEORICAS Y APLIC.
Articulos(SEDE CENTRAL)
Articulos de SEDE CENTRAL
Citación
Devida, Juan Marcelo; Herrera, Facundo Carlos; Daza Millone, Maria Antonieta; Requejo, Felix Gregorio; Pallarola, Diego Andres; Electrochemical Fine-Tuning of the Chemoresponsiveness of Langmuir-Blodgett Graphene Oxide Films; American Chemical Society; ACS Omega; 8; 30; 7-2023; 27566-27575
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