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dc.contributor.author
Juan de Paz, Leornardo
dc.contributor.author
Graf, Daniel Adolfo
dc.contributor.author
Scandizzi, Angel Luis
dc.contributor.author
Guibert, Edgardo Elvio
dc.contributor.author
Rodriguez, Joaquin Valentin
dc.date.available
2017-08-07T18:50:27Z
dc.date.issued
2013-08
dc.identifier.citation
Juan de Paz, Leornardo; Graf, Daniel Adolfo; Scandizzi, Angel Luis; Guibert, Edgardo Elvio; Rodriguez, Joaquin Valentin; A device to record ultra-rapid cooling profiles; Cryo Letters; Cryo-letters; 34; 4; 8-2013; 360-368
dc.identifier.issn
0143-2044
dc.identifier.uri
http://hdl.handle.net/11336/21966
dc.description.abstract
This work deals with the construction and performance of a measuring system capable of estimating temperature at sufficiently high speed (up to 1000 samples/sec). Due to its simple design and the utilization of standard materials, it could serve to recording the cooling profile of ultra-rapid procedures. An immersion device was also developed with the purpose of normalize the penetration speed of the sample in the LN2. The device allows also the comparative analysis of different cooling profiles. The system consists of an immersion device of the sample in the cooling agent, a temperature measurement system developed by Kleihans F and a laptop computer. To test the system, we recorded the cooling profiles of 10 L of distilled water and 6 M glycerol solution, obtaining a cooling rate of 8732 °C.min-1 and 4441 °C.min-1 respectively. Also we determine a cooling rate of 204.012 °C.min-1 during the immersion of the thermocouple assembly in LN2. Although, the same device, with small technical modifications related to the handling of the sample, could be used to evaluate the recovery from LN2 temperature to room temperature (re-warming).
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Cryo Letters
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Cooling
dc.subject
Vitrification
dc.subject.classification
Bioquímica y Biología Molecular
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
A device to record ultra-rapid cooling profiles
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-08-04T13:48:58Z
dc.journal.volume
34
dc.journal.number
4
dc.journal.pagination
360-368
dc.journal.pais
Reino Unido
dc.description.fil
Fil: Juan de Paz, Leornardo. Universidad Nacional de Rosario. Secretaria de Ciencia y Tecnica. Centro Binacional de Investigación en Criobiologia Clinica y Aplicada; Argentina
dc.description.fil
Fil: Graf, Daniel Adolfo. Universidad Nacional de Rosario. Secretaria de Ciencia y Tecnica. Centro Binacional de Investigación en Criobiologia Clinica y Aplicada; Argentina
dc.description.fil
Fil: Scandizzi, Angel Luis. Universidad Nacional de Rosario. Secretaria de Ciencia y Tecnica. Centro Binacional de Investigación en Criobiologia Clinica y Aplicada; Argentina
dc.description.fil
Fil: Guibert, Edgardo Elvio. Universidad Nacional de Rosario. Secretaria de Ciencia y Tecnica. Centro Binacional de Investigación en Criobiologia Clinica y Aplicada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Rodriguez, Joaquin Valentin. Universidad Nacional de Rosario. Secretaria de Ciencia y Tecnica. Centro Binacional de Investigación en Criobiologia Clinica y Aplicada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.journal.title
Cryo-letters
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.cryoletters.org/Abstracts/vol_34_4_2013.htm#360
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