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dc.contributor.author
Molinari, Gabriela Beatriz
dc.contributor.author
Kujawski, Maciej
dc.contributor.author
Scuto, Anna
dc.contributor.author
Soloneski, Sonia Maria Elsa
dc.contributor.author
Larramendy, Marcelo Luis
dc.date.available
2017-08-04T22:20:20Z
dc.date.issued
2012-09
dc.identifier.citation
Molinari, Gabriela Beatriz; Kujawski, Maciej; Scuto, Anna; Soloneski, Sonia Maria Elsa; Larramendy, Marcelo Luis; DNA damage kinetics and apoptosis in ivermectin-treated chinese hamster ovary cells; Wiley; Journal of Applied Toxicology; 33; 11; 9-2012; 1260-1267
dc.identifier.issn
0260-437X
dc.identifier.uri
http://hdl.handle.net/11336/21940
dc.description.abstract
A comet assay was used to analyze DNA damage kinetics in Chinese hamster ovary (CHO-K1) cells induced by antiparasitic ivermectin (IVM) and the IVM-containing technical formulation IvomecW (IVO; 1% IVM). Cells were treated with 50 mg ml–1 IVM and IVO for 80 min, washed and re-incubated in antiparasiticide-free medium for 0–24 h until assayed using the single-cell gel electrophoresis assay (SCGE). Cell viability remained unchanged up to 3 h of incubation. After 6 h of treatment, cell survival decreased up to 75% and 79% in IVM- and IVO-treated cultures, respectively, remaining unchanged within 12–24 h after treatment. For both anthelmintics, biphasic behavior in DNA damage occurred during the incubation time. A time-dependent increase of IVM- and IVO-induced DNA damage was observed within 0 to 3 h after pulse treatment, revealed by a progressive decrease of undamaged cells and an increase in slightly damaged and damaged cells. Finally, a time-dependent decrease in IVM- and IVO-induced DNA damage was revealed by a progressive decrease of slightly damaged cells and the absence of damaged cells simultaneously with an increase in the frequency of undamaged cells during the final 18 h of incubation. Flow cytometry analysis revealed that both compounds are able to induce a marked increase in early and late apoptosis. Based on our observations, we could conclude that the decrease in DNA lesions is mostly related to IVMinduced cytotoxicity rather than attributable to a repair process. Copyright © 2012 John Wiley & Sons, Ltd.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Wiley
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Cho-K1 Cells
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Comet Assay
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Flow Cytometry
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Apoptosis
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Ivermectin
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Ivomecw
dc.subject.classification
Bioquímica y Biología Molecular
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Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
DNA damage kinetics and apoptosis in ivermectin-treated chinese hamster ovary cells
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-08-04T14:00:29Z
dc.journal.volume
33
dc.journal.number
11
dc.journal.pagination
1260-1267
dc.journal.pais
Estados Unidos
dc.journal.ciudad
Hoboken
dc.description.fil
Fil: Molinari, Gabriela Beatriz. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Kujawski, Maciej. City of Hope Comprehensive Cancer Center; Estados Unidos
dc.description.fil
Fil: Scuto, Anna. City of Hope Comprehensive Cancer Center; Estados Unidos
dc.description.fil
Fil: Soloneski, Sonia Maria Elsa. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Larramendy, Marcelo Luis. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.journal.title
Journal of Applied Toxicology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1002/jat.2782
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/jat.2782/abstract


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