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dc.contributor.author
Romero, José Rodolfo
dc.contributor.author
Selva, Juan Pablo
dc.contributor.author
Pessino, Silvina Claudia
dc.contributor.author
Echenique, Carmen Viviana
dc.contributor.author
Garbus, Ingrid
dc.date.available
2017-08-04T19:02:55Z
dc.date.issued
2016-01
dc.identifier.citation
Romero, José Rodolfo; Selva, Juan Pablo; Pessino, Silvina Claudia; Echenique, Carmen Viviana; Garbus, Ingrid; Repetitive sequences in Eragrostis curvula cDNA EST libraries obtained from genotypes with different ploidy; Springer; Biologia Plantarum; 60; 1; 1-2016; 55-67
dc.identifier.issn
0006-3134
dc.identifier.uri
http://hdl.handle.net/11336/21880
dc.description.abstract
Eragrostis curvula (Schrad) Nees (weeping lovegrass) represents an important cultivated forage resource in semiarid regions, and the most useful cultivars are tetraploid and reproduce by pseudogamous diplosporous apomixis. We previously produced a series of genetically related E. curvula lines that provide a suitable system for the identification of gene(s) involved in diplosporous apomixis and ploidy, including a natural apomictic tetraploid (T), a diploid sexual line (D) and a tetraploid sexual plant (C). A collection of ESTs was generated from cDNA libraries obtained from panicles of D, T and C, and leaves of T. The present study aimed to analyze the repetitive content of these four cDNA libraries and further identify and characterize transposable element (TE)-related ESTs. Repetitive sequences were identified though the interface RepeatMasker (RM) using the database Repbase Update and the further classification of transposable elements was performed manually from the RM output. The differential contribution of ESTs with identity to TEs among libraries was further evaluated and such differences were validated through qRT-PCR. We found that the percentage of repetitive content in the leaf cDNA library was almost double that in inflorescence libraries, with retrotransposons contributing most in all libraries. The expression of TE-related ESTs was compared in cDNA samples extracted from D, T and C leaves or inflorescences, revealing that seven mRNAs containing MuDR-like DNA transposons, Gypsy-like and Copia-like retrotransposons were differentially represented according to tissue, reproductive mode or ploidy. The euploid series of the Eragrostis curvula is a useful model to the study the epigenomic changes produced after changes in ploidy. The present work constitutes the first detailed report on repetitive sequences of Eragrostis curvula at the transcriptome level.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Springer
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Diplosporous Apomixis
dc.subject
Weeping Lovegrass
dc.subject
Transposable Elements
dc.subject.classification
Bioquímica y Biología Molecular
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Repetitive sequences in Eragrostis curvula cDNA EST libraries obtained from genotypes with different ploidy
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-07-27T12:27:08Z
dc.journal.volume
60
dc.journal.number
1
dc.journal.pagination
55-67
dc.journal.pais
Países Bajos
dc.journal.ciudad
Dordrecht
dc.description.fil
Fil: Romero, José Rodolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
dc.description.fil
Fil: Selva, Juan Pablo. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
dc.description.fil
Fil: Pessino, Silvina Claudia. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Echenique, Carmen Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; Argentina
dc.description.fil
Fil: Garbus, Ingrid. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur; Argentina
dc.journal.title
Biologia Plantarum
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1007/s10535-015-0569-z
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007/s10535-015-0569-z


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