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dc.contributor.author
Machado, Mizael
dc.contributor.author
Castro, Márcio B.
dc.contributor.author
Wilson, Tais M.
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Gonçalves, Alexandra A. B.
dc.contributor.author
Portiansky, Enrique Leo
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dc.contributor.author
Riet Correa, Franklin
dc.contributor.author
Barros, Severo S.
dc.date.available
2023-11-17T14:25:17Z
dc.date.issued
2022-09
dc.identifier.citation
Machado, Mizael; Castro, Márcio B.; Wilson, Tais M.; Gonçalves, Alexandra A. B.; Portiansky, Enrique Leo; et al.; Poisoning by Nierembergia veitchii: Effects on vascular smooth muscle cells in the pathogenesis of enzootic calcinosis; American College of Veterinary Pathologists; Veterinary Pathology; 59; 5; 9-2022; 814-823
dc.identifier.issn
0300-9858
dc.identifier.uri
http://hdl.handle.net/11336/218364
dc.description.abstract
Vascular mineralization is a hallmark of enzootic calcinosis. Histopathological, ultrastructural, and immunohistochemical investigations were performed on the external carotid arteries of seven sheep naturally poisoned by Nierembergia veitchii. Histologically, moderate to marked hyperplasia of the tunica intima was observed without mineralization. The tunica media exhibited mild to severe mineralization and osteochondroid metaplasia. Sheep with enzootic calcinosis showed arterial overexpression of osteopontin and tissue-nonspecific alkaline phosphatase and immunolabeling for osteonectin and osteocalcin in both intima and media layers of the tested arteries. The main ultrastructural finding in the tunica media was a marked phenotypic change of vascular smooth muscle cells from a contractile phenotype (VSMC-C) into a synthetic phenotype (VSMC-S). In the tunica media, VSMC-S produced matrix and extracellular vesicles, forming mineralizable granules associated with arterial mineralization. VSMC-S were also present in the tunica intima, but matrix and extracellular vesicles and mineralization were not observed. The absence of matrix and extracellular vesicles in the intimal hyperplasia, even in the presence of noncollagenous bone proteins, tissue-nonspecific alkaline phosphatase, and vitamin D receptors, reinforces the hypothesis that the presence of matrix and extracellular vesicles are crucial for the development of vascular mineralization in enzootic calcinosis. It is proposed that the two different VSMC-S phenotypes in calcinosis are due to the expression of at least two genetically different types of these cells induced by the action of 1,25(OH)2D3.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
American College of Veterinary Pathologists
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
ARTERIAL MINERALIZATION
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ARTERIES
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ENZOOTIC CALCINOSIS
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EXOSOMES
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PATHOGENESIS
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TOXICITY
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VASCULAR SMOOTH MUSCLE CELLS
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Otras Ciencias de la Salud
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dc.subject.classification
Ciencias de la Salud
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dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
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dc.title
Poisoning by Nierembergia veitchii: Effects on vascular smooth muscle cells in the pathogenesis of enzootic calcinosis
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2023-11-14T14:30:30Z
dc.journal.volume
59
dc.journal.number
5
dc.journal.pagination
814-823
dc.journal.pais
Estados Unidos
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dc.description.fil
Fil: Machado, Mizael. Instituto Nacional de Investigación Agropecuaria Inia Uruguay; Uruguay
dc.description.fil
Fil: Castro, Márcio B.. Universidade do Brasília; Brasil
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Fil: Wilson, Tais M.. Universidade do Brasília; Brasil
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Fil: Gonçalves, Alexandra A. B.. Universidade do Brasília; Brasil
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Fil: Portiansky, Enrique Leo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata; Argentina
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Fil: Riet Correa, Franklin. Instituto Nacional de Investigación Agropecuaria Inia Uruguay; Uruguay. Universidade Federal da Bahia; Brasil
dc.description.fil
Fil: Barros, Severo S.. Universidade Federal de Santa Maria; Brasil
dc.journal.title
Veterinary Pathology
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1177/03009858221098430
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://journals.sagepub.com/doi/10.1177/03009858221098430
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