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dc.contributor.author
Meffre, D.
dc.contributor.author
Labombarda, Maria Florencia
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Delespierre, B.
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Chastre, A.
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de Nicola, Alejandro Federico
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Stein, D. G.
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Schumacher, Michael
dc.contributor.author
Guennoun, Rachida
dc.date.available
2015-09-28T20:27:53Z
dc.date.issued
2013-02-12
dc.identifier.citation
Meffre, D.; Labombarda, Maria Florencia; Delespierre, B.; Chastre, A.; de Nicola, Alejandro Federico; et al.; Distribution of membrane progesterone receptor alpha in the male mouse and rat brain and its regulation after traumatic brain injury; Pergamon-Elsevier Science Ltd; Neuroscience; 231; 12-2-2013; 111-124
dc.identifier.issn
0306-4522
dc.identifier.uri
http://hdl.handle.net/11336/2180
dc.description.abstract
Progesterone has been shown to exert pleiotropic actions in the brain of both male and females. In particular, after traumatic brain injury (TBI), progesterone has important neuroprotective effects. In addition to intracellular progesterone receptors, membrane receptors of the hormone such as membrane progesterone receptor (mPR) may also be involved in neuroprotection. Three mPR subtypes (mPRα, mPRβ, and mPRγ) have been described and mPRα is best characterized pharmacologically. In the present study we investigated the distribution, cellular localization and the regulation of mPRα in male mouse and rat brain. We showed by reverse transcription-PCR that mPRα is expressed at similar levels in the male and female mouse brain suggesting that its expression may not be influenced by steroid levels. Treatment of males by estradiol or progesterone did not modify the level of expression of mPRα as shown by Western blot analysis. In situhybridization and immunohistochemistry analysis showed a wide expression of mPRα in particular in the olfactory bulb, striatum, cortex, thalamus, hypothalamus, septum, hippocampus and cerebellum. Double immunofluorescence and confocal microscopy analysis showed that mPRα is expressed by neurons but not by oligodendrocytes and astrocytes. In the rat brain, the distribution of mPRα was similar to that observed in mouse brain; and after TBI, mPRα expression was induced in oligodendrocytes, astrocytes and reactive microglia. The wide neuroanatomical distribution of mPRα suggests that this receptor may play a role beyond neuroendocrine and reproductive functions. However, in the absence of injury its role might be restricted to neurons. The induction of mPRα after TBI in microglia, astrocytes and oligodendrocytes, points to a potential role in mediating the modulatory effects of progesterone in inflammation, ion and water homeostasis and myelin repair in the injured brain.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Pergamon-Elsevier Science Ltd
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nd/2.5/ar/
dc.subject
ASTROCYTES
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MEMBRANE PROGESTERONE RECEPTOR (MPR)
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MICROGLIA
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NEURONS
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OLIGODENDROCYTES
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TRAUMATIC BRAIN INJURY (TBI)
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Bioquímica y Biología Molecular
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Medicina Básica
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CIENCIAS MÉDICAS Y DE LA SALUD
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Farmacología y Farmacia
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Medicina Básica
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CIENCIAS MÉDICAS Y DE LA SALUD
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Neurociencias
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Medicina Básica
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CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
Distribution of membrane progesterone receptor alpha in the male mouse and rat brain and its regulation after traumatic brain injury
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2016-03-30 10:35:44.97925-03
dc.identifier.eissn
1873-7544
dc.journal.volume
231
dc.journal.pagination
111-124
dc.journal.pais
Estados Unidos
dc.description.fil
Fil: Meffre, D.. Inserm; Francia
dc.description.fil
Fil: Labombarda, Maria Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
dc.description.fil
Fil: Delespierre, B.. Inserm; Francia
dc.description.fil
Fil: Chastre, A.. Inserm; Francia
dc.description.fil
Fil: de Nicola, Alejandro Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
dc.description.fil
Fil: Stein, D. G.. University of Emory; Estados Unidos
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Fil: Schumacher, Michael. Inserm; Francia
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Fil: Guennoun, Rachida. Inserm; Francia
dc.journal.title
Neuroscience
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0306452212011505
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.neuroscience.2012.11.039
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