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dc.contributor.author
Lobo, Constanza Belén  
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Correa Deza, Maria Alejandra  
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Molina, Rocío Daniela Inés  
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Ferrero, Marcela Alejandra  
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Juárez Tomás, María Silvina  
dc.date.available
2023-11-14T16:06:03Z  
dc.date.issued
2023  
dc.identifier.citation
Removal of polycyclic aromatic compounds by pseudomonas sp. P26 in an immobilized system on organic waste as culture and support medium. Influence of biostimulation with inorganic phosphate; XVIII Congreso Argentino de Microbiología General; Chapadmalal; Argentina; 2023; 72-73  
dc.identifier.uri
http://hdl.handle.net/11336/218077  
dc.description.abstract
Microbial immobilization is a beneficial strategy that can ensure greater efficiency and persistence of microorganisms used in petroleum compound biodegradation processes. Natural organic supports for immobilizing microbial cells are cost-effective, biocompatible and biodegradable. Particularly, walnut shell is a hydrophilic, porous support with high wear resistance. The study objectives were: 1) to evaluate the immobilization of Pseudomonas sp. P26, an environmental bacterium that removes aromatic petroleum compounds, using walnut shell and a low-cost culture medium, and 2) to determine the removal of a mixture of dibenzothiophene (DBT) and polycyclic aromatic hydrocarbons (PAHs) by the immobilized system and planktonic cells, in the presence and absence of an inorganic biostimulant (KH2PO4). Pseudomonas sp. P26 was pre-cultured in LBm broth (24 h, 30°C, 180 rpm) and immobilized in bioreactors containing ground walnut shell (support) and a previously formulated low-cost culture medium (2.5% corn maceration water and 1% crude glycerol). This system was incubated at 30°C for 72 hours without agitation. Viable cultivable cell counts were performed, and metabolic activity was determined using the thiazolyl blue tetrazolium bromide (MTT) reduction technique to MTT-formazan. The removal of a mixture of PAHs (DBT, acenaphthene, fluoranthene, and pyrene; 0.2 mM each) by the immobilized system and control planktonic cultures was determined in JPP broth, with different concentrations of KH2PO4 (0 and 0.46%), after 7 days of incubation at 30°C and 100 rpm. Accumulation of inorganic polyphosphate (polyP) was determined by the colorimetric method of acid hydrolysis and subsequent reaction with phosphomolybdate in the presence of ferrous sulfate. Bioemulsifying activity was assessed by the non-polar solvent mechanical agitation method, and the remaining concentration of contaminants was measured using reverse phase high-performance liquid chromatography. The results of metabolic activity (15% conversion to MTT-formazan) and bacterial viability (1.9 x 107 CFU/g support) indicated that Pseudomonas sp. P26 was effectively immobilized in walnut shells using the formulated culture medium with industrial by-products. The highest removal percentages of DBT (33%), fluoranthene (23%), and pyrene (25%) were observed in the immobilized system compared to planktonic cells. The presence of inorganic phosphate did not significantly affect contaminant removal or bioemulsifying activity in both immobilized and planktonic cultures, while the highest intracellular accumulation of polyP was evidenced in planktonic cultures. The obtained results demonstrate that walnut shells, corn maceration water, and crude glycerol are organic waste materials that can enhance bacterial immobilization systems to be applied in the transformation of petroleum compounds in bioremediation or refining technologies.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Sociedad Argentina de Microbiología General  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
Microbial immobilization  
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Bioremediation  
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Natural carriers  
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Low-cost culture medium  
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Biotecnología Medioambiental  
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Biotecnología del Medio Ambiente  
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INGENIERÍAS Y TECNOLOGÍAS  
dc.title
Removal of polycyclic aromatic compounds by pseudomonas sp. P26 in an immobilized system on organic waste as culture and support medium. Influence of biostimulation with inorganic phosphate  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.type
info:eu-repo/semantics/conferenceObject  
dc.type
info:ar-repo/semantics/documento de conferencia  
dc.date.updated
2023-11-13T15:48:32Z  
dc.journal.pagination
72-73  
dc.journal.pais
Argentina  
dc.journal.ciudad
Capadmalal  
dc.description.fil
Fil: Lobo, Constanza Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina  
dc.description.fil
Fil: Correa Deza, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina  
dc.description.fil
Fil: Molina, Rocío Daniela Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina  
dc.description.fil
Fil: Ferrero, Marcela Alejandra. YPF - Tecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina  
dc.description.fil
Fil: Juárez Tomás, María Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://samige.org.ar/wp-content/uploads/2023/09/Libro-de-Resumenes-Final.pdf  
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Autor  
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Autor  
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Autor  
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Autor  
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Autor  
dc.coverage
Nacional  
dc.type.subtype
Congreso  
dc.description.nombreEvento
XVIII Congreso Argentino de Microbiología General  
dc.date.evento
2023-10-02  
dc.description.ciudadEvento
Chapadmalal  
dc.description.paisEvento
Argentina  
dc.type.publicacion
Book  
dc.description.institucionOrganizadora
Sociedad Argentina de Microbiología General  
dc.source.libro
Libro de Resúmenes: XVIII Congreso Argentino de Microbiología General  
dc.date.eventoHasta
2023-10-05  
dc.type
Congreso