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dc.contributor.author
Lobo, Constanza Belén
dc.contributor.author
Correa Deza, Maria Alejandra
dc.contributor.author
Molina, Rocío Daniela Inés
dc.contributor.author
Ferrero, Marcela Alejandra
dc.contributor.author
Juárez Tomás, María Silvina
dc.date.available
2023-11-14T16:06:03Z
dc.date.issued
2023
dc.identifier.citation
Removal of polycyclic aromatic compounds by pseudomonas sp. P26 in an immobilized system on organic waste as culture and support medium. Influence of biostimulation with inorganic phosphate; XVIII Congreso Argentino de Microbiología General; Chapadmalal; Argentina; 2023; 72-73
dc.identifier.uri
http://hdl.handle.net/11336/218077
dc.description.abstract
Microbial immobilization is a beneficial strategy that can ensure greater efficiency and persistence of microorganisms used in petroleum compound biodegradation processes. Natural organic supports for immobilizing microbial cells are cost-effective, biocompatible and biodegradable. Particularly, walnut shell is a hydrophilic, porous support with high wear resistance. The study objectives were: 1) to evaluate the immobilization of Pseudomonas sp. P26, an environmental bacterium that removes aromatic petroleum compounds, using walnut shell and a low-cost culture medium, and 2) to determine the removal of a mixture of dibenzothiophene (DBT) and polycyclic aromatic hydrocarbons (PAHs) by the immobilized system and planktonic cells, in the presence and absence of an inorganic biostimulant (KH2PO4). Pseudomonas sp. P26 was pre-cultured in LBm broth (24 h, 30°C, 180 rpm) and immobilized in bioreactors containing ground walnut shell (support) and a previously formulated low-cost culture medium (2.5% corn maceration water and 1% crude glycerol). This system was incubated at 30°C for 72 hours without agitation. Viable cultivable cell counts were performed, and metabolic activity was determined using the thiazolyl blue tetrazolium bromide (MTT) reduction technique to MTT-formazan. The removal of a mixture of PAHs (DBT, acenaphthene, fluoranthene, and pyrene; 0.2 mM each) by the immobilized system and control planktonic cultures was determined in JPP broth, with different concentrations of KH2PO4 (0 and 0.46%), after 7 days of incubation at 30°C and 100 rpm. Accumulation of inorganic polyphosphate (polyP) was determined by the colorimetric method of acid hydrolysis and subsequent reaction with phosphomolybdate in the presence of ferrous sulfate. Bioemulsifying activity was assessed by the non-polar solvent mechanical agitation method, and the remaining concentration of contaminants was measured using reverse phase high-performance liquid chromatography. The results of metabolic activity (15% conversion to MTT-formazan) and bacterial viability (1.9 x 107 CFU/g support) indicated that Pseudomonas sp. P26 was effectively immobilized in walnut shells using the formulated culture medium with industrial by-products. The highest removal percentages of DBT (33%), fluoranthene (23%), and pyrene (25%) were observed in the immobilized system compared to planktonic cells. The presence of inorganic phosphate did not significantly affect contaminant removal or bioemulsifying activity in both immobilized and planktonic cultures, while the highest intracellular accumulation of polyP was evidenced in planktonic cultures. The obtained results demonstrate that walnut shells, corn maceration water, and crude glycerol are organic waste materials that can enhance bacterial immobilization systems to be applied in the transformation of petroleum compounds in bioremediation or refining technologies.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Sociedad Argentina de Microbiología General
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Microbial immobilization
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Bioremediation
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Natural carriers
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Low-cost culture medium
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Biotecnología Medioambiental
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Biotecnología del Medio Ambiente
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INGENIERÍAS Y TECNOLOGÍAS
dc.title
Removal of polycyclic aromatic compounds by pseudomonas sp. P26 in an immobilized system on organic waste as culture and support medium. Influence of biostimulation with inorganic phosphate
dc.type
info:eu-repo/semantics/publishedVersion
dc.type
info:eu-repo/semantics/conferenceObject
dc.type
info:ar-repo/semantics/documento de conferencia
dc.date.updated
2023-11-13T15:48:32Z
dc.journal.pagination
72-73
dc.journal.pais
Argentina
dc.journal.ciudad
Capadmalal
dc.description.fil
Fil: Lobo, Constanza Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
dc.description.fil
Fil: Correa Deza, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
dc.description.fil
Fil: Molina, Rocío Daniela Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
dc.description.fil
Fil: Ferrero, Marcela Alejandra. YPF - Tecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Juárez Tomás, María Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://samige.org.ar/wp-content/uploads/2023/09/Libro-de-Resumenes-Final.pdf
dc.conicet.rol
Autor
dc.conicet.rol
Autor
dc.conicet.rol
Autor
dc.conicet.rol
Autor
dc.conicet.rol
Autor
dc.coverage
Nacional
dc.type.subtype
Congreso
dc.description.nombreEvento
XVIII Congreso Argentino de Microbiología General
dc.date.evento
2023-10-02
dc.description.ciudadEvento
Chapadmalal
dc.description.paisEvento
Argentina
dc.type.publicacion
Book
dc.description.institucionOrganizadora
Sociedad Argentina de Microbiología General
dc.source.libro
Libro de Resúmenes: XVIII Congreso Argentino de Microbiología General
dc.date.eventoHasta
2023-10-05
dc.type
Congreso
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