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dc.contributor.author
Alvarez, Maria Soledad
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dc.contributor.author
Núñez, Estefanía
dc.contributor.author
Fuertes Agudo, Marina
dc.contributor.author
Cucarella, Carme
dc.contributor.author
Fernandez Velasco, Maria
dc.contributor.author
Boscá, Lisardo
dc.contributor.author
Vázquez, Jesús
dc.contributor.author
Rossignol, Rodrigue
dc.contributor.author
Martin Sanz, Paloma
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dc.contributor.author
Casado, Marta
dc.date.available
2023-11-13T14:07:23Z
dc.date.issued
2022-11
dc.identifier.citation
Alvarez, Maria Soledad; Núñez, Estefanía; Fuertes Agudo, Marina; Cucarella, Carme; Fernandez Velasco, Maria; et al.; Quantitative Proteomics Analysis Reveals That Cyclooxygenase-2 Modulates Mitochondrial Respiratory Chain Complex IV in Cardiomyocytes; MDPI; International Journal of Molecular Sciences; 23; 21; 11-2022; 1-19
dc.identifier.issn
1661-6596
dc.identifier.uri
http://hdl.handle.net/11336/217891
dc.description.abstract
The biochemical mechanisms of cell injury and myocardial cell death after myocardial infarction remain unresolved. Cyclooxygenase 2 (COX-2), a key enzyme in prostanoid synthesis, is expressed in human ischemic myocardium and dilated cardiomyopathy, but it is absent in healthy hearts. To assess the role of COX-2 in cardiovascular physiopathology, we developed transgenic mice that constitutively express functional human COX-2 in cardiomyocytes under the control of the α-myosin heavy chain promoter. These animals had no apparent phenotype but were protected against ischemia-reperfusion injury in isolated hearts, with enhanced functional recovery and diminished cellular necrosis. To further explore the phenotype of this animal model, we carried out a differential proteome analysis of wild-type vs. transgenic cardiomyocytes. The results revealed a tissue-specific proteomic profile dominated by mitochondrial proteins. In particular, an increased expression of respiratory chain complex IV proteins was observed. This correlated with increased catalytic activity, enhanced respiratory capacity, and increased ATP levels in the heart of COX-2 transgenic mice. These data suggest a new link between COX-2 and mitochondria, which might contribute to the protective cardiac effects of COX-2 against ischemia-reperfusion injury.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
MDPI
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by/2.5/ar/
dc.subject
COX-2
dc.subject
MITOCHONDRIA
dc.subject
PROSTAGLANDINS
dc.subject
RESPIRATORY CAPACITY
dc.subject
TRANSGENIC ANIMALS
dc.subject.classification
Bioquímica y Biología Molecular
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dc.subject.classification
Medicina Básica
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dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
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dc.title
Quantitative Proteomics Analysis Reveals That Cyclooxygenase-2 Modulates Mitochondrial Respiratory Chain Complex IV in Cardiomyocytes
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2023-11-13T10:37:44Z
dc.identifier.eissn
1422-0067
dc.journal.volume
23
dc.journal.number
21
dc.journal.pagination
1-19
dc.journal.pais
Suiza
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dc.journal.ciudad
Basel
dc.description.fil
Fil: Alvarez, Maria Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina
dc.description.fil
Fil: Núñez, Estefanía. No especifíca;
dc.description.fil
Fil: Fuertes Agudo, Marina. Consejo Superior de Investigaciones Científicas; España
dc.description.fil
Fil: Cucarella, Carme. Consejo Superior de Investigaciones Científicas; España
dc.description.fil
Fil: Fernandez Velasco, Maria. Consejo Superior de Investigaciones Científicas; España
dc.description.fil
Fil: Boscá, Lisardo. Consejo Superior de Investigaciones Científicas; España
dc.description.fil
Fil: Vázquez, Jesús. Consejo Superior de Investigaciones Científicas; España
dc.description.fil
Fil: Rossignol, Rodrigue. No especifíca;
dc.description.fil
Fil: Martin Sanz, Paloma. Consejo Superior de Investigaciones Científicas; España
dc.description.fil
Fil: Casado, Marta. Consejo Superior de Investigaciones Científicas; España
dc.journal.title
International Journal of Molecular Sciences
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.3390/ijms232113476
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