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Artículo

Detection of synergistic combinations of Baccharis extracts with Terbinafine against Trichophyton rubrum with high throughput screening synergy assay (HTSS) followed by 3D graphs. Behavior of some of their components

Rodriguez, María VictoriaIcon ; Sortino, Maximiliano AndrésIcon ; Ivancovich, Juan J.; Pellegrino, Jose ManuelIcon ; Favier, Laura Silvina; Raimondi, Marcela P.; Gattuso, Martha Ana; Zacchino, Susana Alicia Stella
Fecha de publicación: 07/2013
Editorial: Elsevier Gmbh
Revista: Phytomedicine
ISSN: 0944-7113
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Bioquímica y Biología Molecular

Resumen

Forty four extracts from nine Baccharis spp. from the Caulopterae section were tested in combination with terbinafine against Trichophyton rubrum with the HTSS assay at six different ratios with the aim of detecting those mixtures that produced a ≥50%statistically significant enhancement of growth inhibition. Since an enhanced effect of a combination respective of its components, does not necessarily indicate synergism, three-dimensional (3D) dose–response surfaces were constructed for each selected pair of extract/antifungal drug with the aid of CombiTool software. Ten extracts showed synergistic or additive combinations which constitutes a 22% hit rate of the extracts submitted to evaluation. Four flavonoids and three ent-clerodanes were detected in the active Baccharis extracts with HPLC/UV/ESI-MS methodology, all of which were tested in combination with terbinafine. Results showed that ent-clerodanes but not flavonoids showed synergistic or additive effects. Among them, bacchotricuneatin A followed by bacrispine showed synergistic effects while hawtriwaic acid showed additive effects.
Palabras clave: High Throughput Screening Synergy Assay , Baccharis Species , Ent-Clerodanes , Flavonoids , Trichophyton Rubrum , Terbinafine , Antifungal , Synergism , Additivism , 3d Surface-Response Curves , Combitool Software
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/21788
DOI: http://dx.doi.org/10.1016/j.phymed.2013.06.015
URL: http://www.sciencedirect.com/science/article/pii/S0944711313002420
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Articulos(CCT - ROSARIO)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - ROSARIO
Citación
Rodriguez, María Victoria; Sortino, Maximiliano Andrés; Ivancovich, Juan J.; Pellegrino, Jose Manuel; Favier, Laura Silvina; et al.; Detection of synergistic combinations of Baccharis extracts with Terbinafine against Trichophyton rubrum with high throughput screening synergy assay (HTSS) followed by 3D graphs. Behavior of some of their components; Elsevier Gmbh; Phytomedicine; 20; 13; 7-2013; 1230-1239
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