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dc.contributor.author
Mojica, María F.
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De La Cadena, Elsa
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Ríos, Rafael
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García Betancur, Juan Carlos
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Díaz, Lorena
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Reyes, Jinnethe
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Hernández Gómez, Cristhian
dc.contributor.author
Radice, Marcela Alejandra
dc.contributor.author
Gales, Ana C.
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Castañeda Méndez, Paulo
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Munita, José M.
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Pallares, Christian José
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Martínez, José R. W.
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Villegas, María Virginia
dc.date.available
2023-11-13T12:24:39Z
dc.date.issued
2022-10
dc.identifier.citation
Mojica, María F.; De La Cadena, Elsa; Ríos, Rafael; García Betancur, Juan Carlos; Díaz, Lorena; et al.; Molecular mechanisms leading to ceftolozane/tazobactam resistance in clinical isolates of Pseudomonas aeruginosa from five Latin American countries; Frontiers Media; Frontiers in Microbiology; 13; 10-2022; 1-8
dc.identifier.uri
http://hdl.handle.net/11336/217847
dc.description.abstract
Objectives: Identify molecular mechanisms responsible for the in vitro non-susceptibility to ceftolozane/tazobactam (TOL) in a group of 158 clinical isolates of Pseudomonas aeruginosa from five Latin American countries collected before the introduction of TOL into the clinical practice. Methods: Clinical isolates of P. aeruginosa (n = 504) were collected between January 2016 and October 2017 from 20 hospitals located in Argentina, Brazil, Chile, Colombia, and Mexico. Minimum inhibitory concentrations (MICs) to TOL were determined by standard broth microdilution and interpreted according to CLSI breakpoints. Initially, production of carbapenemases in TOL non-susceptible isolates was assessed by Rapidec® followed by qPCR to detect blaKPC, blaNDM-1, blaVIM, and blaIMP. Illumina® WGS was performed for isolates in which non-susceptibility to TOL was not mediated by carbapenemases. Results: A total of 158 (31.3%) isolates were non-susceptible to TOL. In 74 (46.8%) of these isolates, non-susceptibility to TOL was explained by the production of at least one carbapenemase. WGS revealed that some isolates carried ESBLs, mutated blaPDC and ampD, associated with decreased susceptibility to TOL. Conclusion: Substitutions found in PDC and carbapenemase production were the most common presumed mechanisms of resistance to TOL detected in this study. This study shows that epidemiological surveillance is warranted to monitor the emergence of novel mechanisms of resistance to TOL that might compromise its clinical utility.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Frontiers Media
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
ANTIBIOTIC RESISTANCE
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CEFTOLOZANE/TAZOBACTAM
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LATIN AMERICA
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MOLECULAR MECHANISMS
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PSEUDOMONAS AERUGINOSA
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Otras Ciencias de la Salud
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Ciencias de la Salud
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CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
Molecular mechanisms leading to ceftolozane/tazobactam resistance in clinical isolates of Pseudomonas aeruginosa from five Latin American countries
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2023-11-10T14:35:20Z
dc.identifier.eissn
1664-302X
dc.journal.volume
13
dc.journal.pagination
1-8
dc.journal.pais
España
dc.description.fil
Fil: Mojica, María F.. Case Western Reserve University; Estados Unidos
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Fil: De La Cadena, Elsa. Universidad El Bosque;
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Fil: Ríos, Rafael. Universidad El Bosque;
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Fil: García Betancur, Juan Carlos. Universidad El Bosque;
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Fil: Díaz, Lorena. Universidad El Bosque;
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Fil: Reyes, Jinnethe. Universidad El Bosque;
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Fil: Hernández Gómez, Cristhian. Universidad El Bosque;
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Fil: Radice, Marcela Alejandra. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
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Fil: Gales, Ana C.. Universidade Federal de Sao Paulo; Brasil
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Fil: Castañeda Méndez, Paulo. Fundacion Clinica Medica Sur; México
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Fil: Munita, José M.. Universidad del Desarrollo; Chile
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Fil: Pallares, Christian José. Universidad El Bosque;
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Fil: Martínez, José R. W.. Universidad del Desarrollo; Chile
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Fil: Villegas, María Virginia. Universidad El Bosque;
dc.journal.title
Frontiers in Microbiology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.3389/fmicb.2022.1035609
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