Artículo
Increased levels of plasma interleukin-6 soluble receptor in patients with essential thrombocythemia
Marta, Rosana Fernanda
; Goette, Nora Paula
; Lev, Paola Roxana
; Heller, Paula Graciela
; Kornblihtt, Laura Inés; Vassallu, Patricia; Glembotski, Ana; Pirola, Carlos José
; Molinas, Felisa Concepción
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Fecha de publicación:
12/2004
Editorial:
Ferrata Storti Foundation
Revista:
Haematologica
ISSN:
0390-6078
e-ISSN:
1592-8721
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Background and Objectives. The pathogenesis of essential thrombocythemia (ET), a disease characterized by megakaryocyte hyperplasia and persistent thrombocytosis, is not completely clarified. Interleukin-6 (IL-6), one of the cytokines related to megakaryocytic development, exerts its effect through binding to a cell surface receptor, IL-6Ra, and a signal transducing unit, gp130. Interestingly, the soluble form of the IL-6Ra, IL-6sR, is an agonist for IL-6 activity. In order to evaluate the possible participation of IL-6sR in ET we measured its levels in plasma, platelets and in the supernatant of a mononuclear cell culture. We also evaluated IL-6R on leukocyte membrane and IL-6R/IL-6sR mRNA expression in mononuclear cells. Design and Methods. Fifty-five patients with ET were evaluated. IL-6sR and IL-6 were measured by an ELISA technique. Mononuclear cells were cultured for 48 hr and IL-6sR released into the supernatant was measured. IL-6R on leukocyte surfaces was evaluated by flow cytometry. IL-6R and IL-6sR mRNA levels were assessed by semi-quantitative reverse transcription polymerase chain reaction. Results. Plasma IL-6sR levels were increased while intraplatelet levels were low in untreated ET patients. Plasma levels decreased during treatment. Non-stimulated mononuclear cells from ET patients released greater amounts of IL-6sR than did cells from normal controls in 48-hour culture. No abnormality was found in IL-6R or IL-6sR mRNA expression by mononuclear ET cells. IL-6R on leukocyte surfaces was normal. Interpretations and Conclusions. Increased plasma IL-6sR levels might have a role in the abnormal megakaryocytic proliferation seen in ET patients, while platelets and mononuclear cells could be the source of the above-mentioned high levels of plasma IL6sR.
Palabras clave:
ILR6 SOLUBLE
,
ESSENTIAL THROMBOCYTHOPENIA
,
MYELOPROLIFERATIVE DISEASES
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Identificadores
Colecciones
Articulos(IDIM)
Articulos de INST.DE INVEST.MEDICAS
Articulos de INST.DE INVEST.MEDICAS
Citación
Marta, Rosana Fernanda; Goette, Nora Paula; Lev, Paola Roxana; Heller, Paula Graciela; Kornblihtt, Laura Inés; et al.; Increased levels of plasma interleukin-6 soluble receptor in patients with essential thrombocythemia; Ferrata Storti Foundation; Haematologica; 89; 12-2004; 657-663
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