Artículo
Host Cell Oxidative Stress Promotes Intracellular Fluoroquinolone Persisters of Streptococcus pneumoniae
Hernandez Morfa, Mirelys
; Reinoso Vizcaino, Nicolas Martin
; Olivero, Nadia Belén
; Zappia, Victoria Eugenia; Cortes, Paulo R.; Jaime, Andrea; Echenique, Jose Ricardo
Fecha de publicación:
11/2022
Editorial:
American Society for Microbiology
Revista:
Microbiology Spectrum
ISSN:
2165-0497
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Bacterial persisters represent a small subpopulation that tolerates high antibiotic concentrations without acquiring heritable resistance, and it may be generated by environmental factors. Here, we report the first antibiotic persistence mechanism in Streptococcus pneumoniae, which is induced by oxidative stress conditions and allows the pneumococcus to survive in the presence of fluoroquinolones. We demonstrated that fluoroquinolone persistence is prompted by both the impact of growth arrest and the oxidative stress response induced by H2O2 in bacterial cells. This process protected pneumococci against the deleterious effects of high ROS levels induced by fluoroquinolones. Importantly, S. pneumoniae develops persistence during infection, and is dependent on the oxidative stress status of the host cells, indicating that its transient intracellular life contributes to this mechanism. Furthermore, our findings suggest persistence may influence the outcome of antibiotic therapy and be part of a multistep mechanism in the evolution of fluoroquinolone resistance.
Palabras clave:
CIPROFLOXACIN
,
FLUOROQUINOLONES
,
HOST-PATHOGEN INTERACTIONS
,
INTRACELLULAR SURVIVAL
,
LEVOFLOXACIN
,
MACROPHAGES
,
MOXIFLOXACIN
,
NEUTROPHILS
,
OXIDATIVE STRESS
,
PATHOGEN INTERACTIONS
,
PERSISTENCE
,
PNEUMOCYTES
,
STREPTOCOCCUS PNEUMONIAE
,
STRESS RESPONSE
,
TRIGGERED-PERSISTENCE
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Identificadores
Colecciones
Articulos(CIBICI)
Articulos de CENTRO DE INV.EN BIOQUI.CLINICA E INMUNOLOGIA
Articulos de CENTRO DE INV.EN BIOQUI.CLINICA E INMUNOLOGIA
Citación
Hernandez Morfa, Mirelys; Reinoso Vizcaino, Nicolas Martin; Olivero, Nadia Belén; Zappia, Victoria Eugenia; Cortes, Paulo R.; et al.; Host Cell Oxidative Stress Promotes Intracellular Fluoroquinolone Persisters of Streptococcus pneumoniae; American Society for Microbiology; Microbiology Spectrum; 10; 6; 11-2022; 1-14
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