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dc.contributor.author
Perez Dorado, Inmaculada
dc.contributor.author
Bortolotti, Ana
dc.contributor.author
Cortez, Nestor Ricardo
dc.contributor.author
Hermoso, Juan A.
dc.date.available
2017-08-01T17:56:59Z
dc.date.issued
2013-01
dc.identifier.citation
Perez Dorado, Inmaculada; Bortolotti, Ana; Cortez, Nestor Ricardo; Hermoso, Juan A.; Structural and Phylogenetic Analysis of Rhodobacter capsulatus NifF: Uncovering General Features of Nitrogen-fixation (nif)-Flavodoxins; Molecular Diversity Preservation International; International Journal of Molecular Sciences; 14; 1; 1-2013; 1152-1163
dc.identifier.issn
1422-0067
dc.identifier.uri
http://hdl.handle.net/11336/21728
dc.description.abstract
Analysis of the crystal structure of NifF from Rhodobacter capsulatus and its homologues reported so far reflects the existence of unique structural features in nif flavodoxins: a leucine at the re face of the isoalloxazine, an eight-residue insertion at the C-terminus of the 50’s loop and a remarkable difference in the electrostatic potential surface with respect to non-nif flavodoxins. A phylogenetic study on 64 sequences from 52 bacterial species revealed four clusters, including different functional prototypes, correlating the previously defined as “short-chain” with the firmicutes flavodoxins and the “long-chain” with gram-negative species. The comparison of Rhodobacter NifF structure with other bacterial flavodoxin prototypes discloses the concurrence of specific features of these functional electron donors to nitrogenase.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Molecular Diversity Preservation International
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Flavodoxin
dc.subject
Nitrogen Fixation
dc.subject
Crystal Structure
dc.subject
Phylogenetic Analysis
dc.subject.classification
Biofísica
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Structural and Phylogenetic Analysis of Rhodobacter capsulatus NifF: Uncovering General Features of Nitrogen-fixation (nif)-Flavodoxins
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-07-28T18:03:26Z
dc.journal.volume
14
dc.journal.number
1
dc.journal.pagination
1152-1163
dc.journal.pais
Suiza
dc.journal.ciudad
Basilea
dc.description.fil
Fil: Perez Dorado, Inmaculada. Consejo Superior de Investigaciones Cientificas; España
dc.description.fil
Fil: Bortolotti, Ana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
dc.description.fil
Fil: Cortez, Nestor Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
dc.description.fil
Fil: Hermoso, Juan A.. Consejo Superior de Investigaciones Cientificas; España
dc.journal.title
International Journal of Molecular Sciences
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.3390/ijms14011152
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.mdpi.com/1422-0067/14/1/1152


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  • Articulos(IBR) [371]
    Articulos de INST.DE BIOLOGIA MOLECULAR Y CELULAR DE ROSARIO

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