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dc.contributor.author
Pellegrini, María Celeste
dc.contributor.author
Okada, Elena
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Gonzalez, Ramon Alejandro
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Ponce, Alejandra Graciela
dc.date.available
2023-11-07T12:51:24Z
dc.date.issued
2021-11
dc.identifier.citation
Pellegrini, María Celeste; Okada, Elena; Gonzalez, Ramon Alejandro; Ponce, Alejandra Graciela; Prevalence of Escherichia coli strains in horticultural farms from Argentina: antibiotic resistance, biofilm formation, and phylogenetic affiliation; Springer Heidelberg; Environmental Science and Pollution Research; 29; 16; 11-2021; 23225-23236
dc.identifier.issn
0944-1344
dc.identifier.uri
http://hdl.handle.net/11336/217265
dc.description.abstract
Escherichia coli is the bacteria most commonly used as an indicator of fecal contamination in agricultural environments. Moreover, E. coli is categorized as a priority pathogen due to its widespread antibiotic resistance. This study aimed to characterize E. coli strains isolated from 10 horticultural farms. Isolates were obtained from samples of vegetable crops (n = 62), the surrounding soil (n = 62), poultry litter (n = 8), and groundwater (n = 6). Phyllo-grouping assignment was performed on the total of E. coli isolates. Antibiograms and quantification of the minimal inhibitory concentration (MIC) were performed with antibiotics commonly used in humans. Biofilm formation capacity was studied by quantifying cells attached to culture tubes. Overall, 21 E. coli isolates were obtained. Three phylogenetic groups (A, B1, and C) and two Escherichia clade IV and IV–V were identified in the collection by polymerase chain reaction. Sixty-seven percent of the E. coli isolates were resistant to amoxicillin-clavulanic acid and/or ampicillin. Amoxicillin MIC values ranged from 11.9 to >190.5 µg/mL and ampicillin MIC values ranged from 3 to >190.5 µg/mL. All the E. coli isolates, resistant and non-resistant, had biofilm forming capacity. The presence of phenotypic resistance on fresh produce and environmental matrices could present significant opportunities for contamination that result in health risks for consumers. To the authors’ best knowledge, this is the first environmental assessment of resistant E. coli occurrence in horticultural farms in South America.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Springer Heidelberg
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
ANTIBIOTICS
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BIOFILM
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ESCHERICHIA COLI
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GROUNDWATER
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HORTICULTURAL ENVIRONMENTS
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PHYLOGROUP
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POULTRY LITTER
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RESISTANCE
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SOIL
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Biología Celular, Microbiología
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
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Otras Ingeniería Química
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Ingeniería Química
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INGENIERÍAS Y TECNOLOGÍAS
dc.title
Prevalence of Escherichia coli strains in horticultural farms from Argentina: antibiotic resistance, biofilm formation, and phylogenetic affiliation
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2023-11-06T09:54:13Z
dc.journal.volume
29
dc.journal.number
16
dc.journal.pagination
23225-23236
dc.journal.pais
Alemania
dc.description.fil
Fil: Pellegrini, María Celeste. Instituto En Ciencia y Tecnología de Alimentos y Ambiente ; Facultad de Ingenieria ; Universidad Nacional de Mar del Plata; . Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina
dc.description.fil
Fil: Okada, Elena. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina
dc.description.fil
Fil: Gonzalez, Ramon Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible - Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina
dc.description.fil
Fil: Ponce, Alejandra Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina. Instituto En Ciencia y Tecnología de Alimentos y Ambiente ; Facultad de Ingenieria ; Universidad Nacional de Mar del Plata;
dc.journal.title
Environmental Science and Pollution Research
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1007/s11356-021-17523-1
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