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dc.contributor.author
Akhgar, Christopher K.
dc.contributor.author
Ebner, Julian
dc.contributor.author
Alcaraz, Mirta R.
dc.contributor.author
Kopp, Julian
dc.contributor.author
Goicoechea, Hector Casimiro
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dc.contributor.author
Spadiut, Oliver
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Schwaighofer, Andreas
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Lendl, Bernhard
dc.date.available
2023-11-03T18:55:23Z
dc.date.issued
2022-08
dc.identifier.citation
Akhgar, Christopher K.; Ebner, Julian; Alcaraz, Mirta R.; Kopp, Julian; Goicoechea, Hector Casimiro; et al.; Application of Quantum Cascade Laser-Infrared Spectroscopy and Chemometrics for In-Line Discrimination of Coeluting Proteins from Preparative Size Exclusion Chromatography; American Chemical Society; Analytical Chemistry; 94; 32; 8-2022; 11192-11200
dc.identifier.issn
0003-2700
dc.identifier.uri
http://hdl.handle.net/11336/217029
dc.description.abstract
An external-cavity quantum cascade laser (EC-QCL)-based flow-through mid-infrared (IR) spectrometer was placed in line with a preparative size exclusion chromatography system to demonstrate real-time analysis of protein elutions with strongly overlapping chromatographic peaks. Two different case studies involving three and four model proteins were performed under typical lab-scale purification conditions. The large optical path length (25 μm), high signal-to-noise ratios, and wide spectral coverage (1350 to 1750 cm-1) of the QCL-IR spectrometer allow for robust spectra acquisition across both the amide I and II bands. Chemometric analysis by self-modeling mixture analysis and multivariate curve resolution enabled accurate quantitation and structural fingerprinting across the protein elution transient. The acquired concentration profiles were found to be in excellent agreement with the off-line high-performance liquid chromatography reference analytics performed on the collected effluent fractions. These results demonstrate that QCL-IR detectors can be used effectively for in-line, real-time analysis of protein elutions, providing critical quality attribute data that are typically only accessible through time-consuming and resource-intensive off-line methods.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
American Chemical Society
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by/2.5/ar/
dc.subject
Amides
dc.subject
Size exclusion chromatography
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Chemometrics
dc.subject
,Chromatography
dc.subject.classification
Química Analítica
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dc.subject.classification
Ciencias Químicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
Application of Quantum Cascade Laser-Infrared Spectroscopy and Chemometrics for In-Line Discrimination of Coeluting Proteins from Preparative Size Exclusion Chromatography
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2023-11-02T14:41:15Z
dc.journal.volume
94
dc.journal.number
32
dc.journal.pagination
11192-11200
dc.journal.pais
Estados Unidos
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dc.journal.ciudad
Washington
dc.description.fil
Fil: Akhgar, Christopher K.. Vienna University of Technology; Austria
dc.description.fil
Fil: Ebner, Julian. Vienna University of Technology; Austria
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Fil: Alcaraz, Mirta R.. Institute Of Chemical Technologies And Analytics; Austria
dc.description.fil
Fil: Kopp, Julian. Vienna University of Technology; Austria
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Fil: Goicoechea, Hector Casimiro. Universidad Nacional del Litoral; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
dc.description.fil
Fil: Spadiut, Oliver. Vienna University of Technology; Austria
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Fil: Schwaighofer, Andreas. Vienna University of Technology; Austria
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Fil: Lendl, Bernhard. Vienna University of Technology; Austria
dc.journal.title
Analytical Chemistry
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://pubs.acs.org/doi/10.1021/acs.analchem.2c01542
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1021/acs.analchem.2c01542
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