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dc.contributor.author
Gallo, Ana Laura  
dc.contributor.author
Soler, María Florencia  
dc.contributor.author
Pellizas, Claudia Gabriela  
dc.contributor.author
Vélez, María Laura  
dc.date.available
2023-10-31T19:15:49Z  
dc.date.issued
2022-08  
dc.identifier.citation
Gallo, Ana Laura; Soler, María Florencia; Pellizas, Claudia Gabriela; Vélez, María Laura; Polysaccharide extracts from mycelia of Ganoderma australe: effect on dendritic cell immunomodulation and antioxidant activity; Springer; Journal of Food Measurement and Characterization; 16; 4; 8-2022; 3251-3262  
dc.identifier.issn
2193-4126  
dc.identifier.uri
http://hdl.handle.net/11336/216713  
dc.description.abstract
Many fungi have inner-polysaccharides (IPS) that can modulate the immune response and/or have antioxidant activity. Ganoderma australe, a species from Patagonia, is a likely candidate to produce bioactive IPS. Our aims were to assess the ability of its IPS to induce maturation and activation of dendritic cells (DCs), to determine their antioxidant activity, and to evaluate the influence of the chemical composition on the bioactivity of the IPS. Mycelia were cultivated in different liquid culture media (M1, M2, and AG), and polysaccharides were extracted with hot water. Mice bone marrow-derived DCs were exposed to the different IPS, and the percentage of cells expressing CD86, MHC II, and IL-12 was determined. The antioxidant activity was evaluated through four assays. Results showed that the chemical composition of the three extracts was different. Glucose, mannose, and galactose prevail in all the extracts, although in different proportions. IPS from mycelia growing in M1 (IPS_M1) significantly increased the expression levels of MHC II and CD86 in a concentration-dependent manner, and the same tendency was found for IL-12 production. This IPS extract was mainly composed of glucose. Only IPS obtained from mycelia growing in M2 (IPS_M2) showed significant antioxidant activity, possibly related to the presence of a greater amount of phenolic compounds in this extract. This is the first study reporting the ability of aqueous extracts of mycelial polysaccharides from G. australe to maturate and activate mouse DCs, as well as to prevent oxidation, encouraging further studies of the immunomodulatory and antioxidant effects of these compounds.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Springer  
dc.rights
info:eu-repo/semantics/restrictedAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
ANTIOXIDANT ACTIVITY  
dc.subject
CARBOHYDRATES  
dc.subject
GANODERMA AUSTRALE  
dc.subject
GLUCOSE  
dc.subject
IMMUNOMODULATION  
dc.subject
MONOSACCHARIDES  
dc.subject.classification
Biología Celular, Microbiología  
dc.subject.classification
Ciencias Biológicas  
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS  
dc.title
Polysaccharide extracts from mycelia of Ganoderma australe: effect on dendritic cell immunomodulation and antioxidant activity  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2023-10-31T17:49:22Z  
dc.identifier.eissn
2193-4134  
dc.journal.volume
16  
dc.journal.number
4  
dc.journal.pagination
3251-3262  
dc.journal.pais
Alemania  
dc.journal.ciudad
Berlín  
dc.description.fil
Fil: Gallo, Ana Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Centro de Investigación y Extensión Forestal Andino Patagónico; Argentina  
dc.description.fil
Fil: Soler, María Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina  
dc.description.fil
Fil: Pellizas, Claudia Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina  
dc.description.fil
Fil: Vélez, María Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Patagonia "San Juan Bosco"; Argentina. Centro de Investigación y Extensión Forestal Andino Patagónico; Argentina  
dc.journal.title
Journal of Food Measurement and Characterization  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1007/s11694-022-01444-9  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007/s11694-022-01444-9