Artículo
Ca2+ signalling system initiated by endoplasmic reticulum stress stimulates PERK activation
Feliziani, Constanza
; Fernández, Macarena
; Quassollo Infanzon, Gonzalo Emiliano
; Holstein, Deborah; Bairo, Sebastián Manuel; Paton, James C.; Paton, Adrienne W.; de Batista, Juan; Lechleiter, James D.; Bollo, Mariana Ines
Fecha de publicación:
09/2022
Editorial:
Elsevier
Revista:
Cell Calcium
ISSN:
0143-4160
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
The accumulation of unfolded proteins within the Endoplasmic Reticulum (ER) activates a signal transduction pathway termed the unfolded protein response (UPR), which attempts to restore ER homoeostasis. If this cannot be done, UPR signalling ultimately induces apoptosis. Ca2+ depletion in the ER is a potent inducer of ER stress. Despite the ubiquity of Ca2+ as an intracellular messenger, the precise mechanism(s) by which Ca2+ release affects the UPR remains unknown. Tethering a genetically encoded Ca2+ indicator (GCamP6) to the ER membrane revealed novel Ca2+ signalling events initiated by Ca2+ microdomains in human astrocytes under ER stress, induced by tunicamycin (Tm), an N-glycosylation inhibitor, as well as in a cell model deficient in all three inositol triphosphate receptor isoforms. Pharmacological and molecular studies indicate that these local events are mediated by translocons and that the Ca2+ microdomains impact (PKR)-like-ER kinase (PERK), an UPR sensor, activation. These findings reveal the existence of a Ca2+ signal mechanism by which stressor-mediated Ca2+ release regulates ER stress.
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Articulos(INIMEC - CONICET)
Articulos de INSTITUTO DE INV. MEDICAS MERCEDES Y MARTIN FERREYRA
Articulos de INSTITUTO DE INV. MEDICAS MERCEDES Y MARTIN FERREYRA
Citación
Feliziani, Constanza; Fernández, Macarena; Quassollo Infanzon, Gonzalo Emiliano; Holstein, Deborah; Bairo, Sebastián Manuel; et al.; Ca2+ signalling system initiated by endoplasmic reticulum stress stimulates PERK activation; Elsevier; Cell Calcium; 106; 9-2022; 1-16
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