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dc.contributor.author
Navarrete, Felipe A.
dc.contributor.author
García Vázquez, Francisco A.
dc.contributor.author
Alvau, Antonio
dc.contributor.author
Escoffier, Jessica
dc.contributor.author
Krapf, Dario
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dc.contributor.author
Sánchez Cárdenas, Claudia
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Salicioni, Ana M.
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Darszon, Alberto
dc.contributor.author
Visconti, Pablo E.
dc.date.available
2017-07-31T20:46:31Z
dc.date.issued
2015-04
dc.identifier.citation
Navarrete, Felipe A.; García Vázquez, Francisco A.; Alvau, Antonio; Escoffier, Jessica; Krapf, Dario; et al.; Biphasic Role of Calcium in Mouse Sperm Capacitation Signaling Pathways; Wiley; Journal Of Cellular Physiology; 230; 8; 4-2015; 1758-1769
dc.identifier.issn
0021-9541
dc.identifier.uri
http://hdl.handle.net/11336/21651
dc.description.abstract
Mammalian sperm acquire fertilizing ability in the female tract in a process known as capacitation. At the molecular level, capacitation is associated with up-regulation of a cAMP-dependent pathway, changes in intracellular pH, intracellular Ca2+, and an increase in tyrosine phosphorylation. How these signaling systems interact during capacitation is not well understood. Results presented in this study indicate that Ca2+ ions have a biphasic role in the regulation of cAMP-dependent signaling. Media without added Ca2+ salts (nominal zero Ca2+) still contain micromolar concentrations of this ion. Sperm incubated in this medium did not undergo PKA activation or the increase in tyrosine phosphorylation suggesting that these phosphorylation pathways require Ca2+. However, chelation of the extracellular Ca2+ traces by EGTA induced both cAMP-dependent phosphorylation and the increase in tyrosine phosphorylation. The EGTA effect in nominal zero Ca2+ media was mimicked by two calmodulin antagonists, W7 and calmidazolium, and by the calcineurin inhibitor cyclosporine A. These results suggest that Ca2+ ions regulate sperm cAMP and tyrosine phosphorylation pathways in a biphasic manner and that some of its effects are mediated by calmodulin. Interestingly, contrary to wild-type mouse sperm, sperm from CatSper1 KO mice underwent PKA activation and an increase in tyrosine phosphorylation upon incubation in nominal zero Ca2+ media. Therefore, sperm lacking Catsper Ca2+ channels behave as wild-type sperm incubated in the presence of EGTA. This latter result suggests that Catsper transports the Ca2+ involved in the regulation of cAMP-dependent and tyrosine phosphorylation pathways required for sperm capacitation.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Wiley
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Sperm Capacitation
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Calcium
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Pka
dc.subject.classification
Biología Reproductiva
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dc.subject.classification
Ciencias Biológicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
Biphasic Role of Calcium in Mouse Sperm Capacitation Signaling Pathways
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2016-11-24T19:36:49Z
dc.journal.volume
230
dc.journal.number
8
dc.journal.pagination
1758-1769
dc.journal.pais
Estados Unidos
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dc.journal.ciudad
Hoboken
dc.description.fil
Fil: Navarrete, Felipe A.. University of Massachusetts; Estados Unidos
dc.description.fil
Fil: García Vázquez, Francisco A.. University of Massachusetts; Estados Unidos. Universidad de Murcia; España
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Fil: Alvau, Antonio. University of Massachusetts; Estados Unidos
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Fil: Escoffier, Jessica. University of Massachusetts; Estados Unidos
dc.description.fil
Fil: Krapf, Dario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
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Fil: Sánchez Cárdenas, Claudia. Universidad Nacional Autónoma de México; México
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Fil: Salicioni, Ana M.. University of Massachusetts; Estados Unidos
dc.description.fil
Fil: Darszon, Alberto. Universidad Nacional Autónoma de México; México
dc.description.fil
Fil: Visconti, Pablo E.. University of Massachusetts; Estados Unidos
dc.journal.title
Journal Of Cellular Physiology
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1002/jcp.24873
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/jcp.24873/abstract
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4752735/
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