Mostrar el registro sencillo del ítem

dc.contributor.author
Pellegrini Malpiedi, Luciana  
dc.contributor.author
Diaz, César A.  
dc.contributor.author
Nerli, Bibiana Beatriz  
dc.contributor.author
Pessoa, Adalberto  
dc.date.available
2017-07-28T20:24:35Z  
dc.date.issued
2013-06  
dc.identifier.citation
Pellegrini Malpiedi, Luciana; Diaz, César A.; Nerli, Bibiana Beatriz; Pessoa, Adalberto; Single-chain antibody fragments: Purification methodologies; Elsevier; Process Biochemistry; 48; 8; 6-2013; 1242-1251  
dc.identifier.issn
1359-5113  
dc.identifier.uri
http://hdl.handle.net/11336/21592  
dc.description.abstract
At present, single-chain variable fragments (scFv) of antibodies are considered one of the most important tools in human therapies. Wide applications of antibodies are being exploited in different medical, pharmaceutical and research areas. These molecules maintain the same binding functionality that full length antibodies but possess several advantageous features as quickness to penetrate the tissues, easy manipulation, fast elimination of their immunocomplex and the possibility of being produced in simple expression systems like bacteria and yeast. The increasing demand in antibody based methodologies is driving advances in the production and purification of genetically engineered antibodies and antibody fragments. While advances in expression systems allow the production of high titers of antibodies, there exist some limits imposed by the downstream methodologies which are not efficient enough to ensure their industrialization. The main aim of this review is to highlight the principal characteristics of single-chain variable fragments of antibodies addressing advances and perspectives on scFv purification.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Elsevier  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/  
dc.subject
Immunoglobulin  
dc.subject
Single-Chain Variable Fragment  
dc.subject
First Capture  
dc.subject
Platform Methodology  
dc.subject.classification
Otras Ciencias Químicas  
dc.subject.classification
Ciencias Químicas  
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS  
dc.title
Single-chain antibody fragments: Purification methodologies  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2017-07-28T18:02:04Z  
dc.journal.volume
48  
dc.journal.number
8  
dc.journal.pagination
1242-1251  
dc.journal.pais
Países Bajos  
dc.journal.ciudad
Amsterdam  
dc.description.fil
Fil: Pellegrini Malpiedi, Luciana. Universidade de Sao Paulo; Brasil. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina  
dc.description.fil
Fil: Diaz, César A.. Universidade de Sao Paulo; Brasil  
dc.description.fil
Fil: Nerli, Bibiana Beatriz. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina  
dc.description.fil
Fil: Pessoa, Adalberto. Universidade de Sao Paulo; Brasil  
dc.journal.title
Process Biochemistry  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.procbio.2013.06.008  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S135951131300278X