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dc.contributor.author
Tomat, David Damian
dc.contributor.author
Mercanti, Diego Javier
dc.contributor.author
Balague, Claudia Elisabeht
dc.contributor.author
Quiberoni, Andrea del Lujan
dc.date.available
2017-07-28T19:57:12Z
dc.date.issued
2013-04
dc.identifier.citation
Tomat, David Damian; Mercanti, Diego Javier; Balague, Claudia Elisabeht; Quiberoni, Andrea del Lujan; Phage biocontrol of enteropathogenic and Shiga toxin-producing Escherichia coli during milk fermentation; Wiley; Letters In Applied Microbiology; 57; 1; 4-2013; 3-10
dc.identifier.issn
0266-8254
dc.identifier.uri
http://hdl.handle.net/11336/21570
dc.description.abstract
Two bacteriophages, isolated from faeces, were assayed as biocontrol agents of pathogenic Escherichia coli during milk fermentation. Phage DT1 was tested on the strain E. coli DH5a, one enteropathogenic E. coli (EPEC) strain and one Shiga toxigenic E. coli O157:H7 (STEC) strain. Phage DT6 was tested on two STEC strains (O157:H7 and non-O157). One additional assay was performed by using a cocktail of both phages against the O157:H7 STEC strain. Streptococcus thermophilus 10-C, the strain used as lactic starter, reached 109 CFU ml 1 after 4 h, while pH values fell to 45 after 8 h, regardless of the presence of E. coli strains and/or phages. In absence of phages, E. coli strains reached 4–6 log CFU ml 1 at 5–6 h. Escherichia coli DH5a and O157:H7 STEC strains were rapidly and completely inactivated by phage DT1 and phage cocktail, respectively, while O157:H7 STEC was completely inactivated either by DT1 or by DT6, after 8 h. The EPEC strain was not detected at 1 h (<10 CFU ml 1 ) but grew afterwards, though at lower rates than without phage. For non-O157:H7 STEC, reductions lower than 1 log CFU ml 1 were observed for all sampling times. Phages DT1 and DT6, either individually or as a cocktail, effectively reduce O157:H7 STEC counts during milk fermentation, without compromising the starter culture performance.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Wiley
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Escherichia Coli O157:H7 Stec
dc.subject
Bacteriophage
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Phage Biocontrol
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Milk Fermentation
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Otros Tópicos Biológicos
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Phage biocontrol of enteropathogenic and Shiga toxin-producing Escherichia coli during milk fermentation
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-07-24T12:50:19Z
dc.journal.volume
57
dc.journal.number
1
dc.journal.pagination
3-10
dc.journal.pais
Estados Unidos
dc.journal.ciudad
Hoboken
dc.description.fil
Fil: Tomat, David Damian. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Mercanti, Diego Javier. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Balague, Claudia Elisabeht. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
dc.description.fil
Fil: Quiberoni, Andrea del Lujan. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.journal.title
Letters In Applied Microbiology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1111/lam.12074
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/lam.12074/abstract


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