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dc.contributor.author Tomat, David Damian
dc.contributor.author Mercanti, Diego Javier
dc.contributor.author Balague, Claudia Elisabeht
dc.contributor.author Quiberoni, Andrea del Lujan
dc.date.available 2017-07-28T19:57:12Z
dc.date.issued 2013-04
dc.identifier.citation Tomat, David Damian; Mercanti, Diego Javier; Balague, Claudia Elisabeht; Quiberoni, Andrea del Lujan; Phage biocontrol of enteropathogenic and Shiga toxin-producing Escherichia coli during milk fermentation; Wiley; Letters In Applied Microbiology; 57; 1; 4-2013; 3-10
dc.identifier.issn 0266-8254
dc.identifier.uri http://hdl.handle.net/11336/21570
dc.description.abstract Two bacteriophages, isolated from faeces, were assayed as biocontrol agents of pathogenic Escherichia coli during milk fermentation. Phage DT1 was tested on the strain E. coli DH5a, one enteropathogenic E. coli (EPEC) strain and one Shiga toxigenic E. coli O157:H7 (STEC) strain. Phage DT6 was tested on two STEC strains (O157:H7 and non-O157). One additional assay was performed by using a cocktail of both phages against the O157:H7 STEC strain. Streptococcus thermophilus 10-C, the strain used as lactic starter, reached 109 CFU ml 1 after 4 h, while pH values fell to 45 after 8 h, regardless of the presence of E. coli strains and/or phages. In absence of phages, E. coli strains reached 4–6 log CFU ml 1 at 5–6 h. Escherichia coli DH5a and O157:H7 STEC strains were rapidly and completely inactivated by phage DT1 and phage cocktail, respectively, while O157:H7 STEC was completely inactivated either by DT1 or by DT6, after 8 h. The EPEC strain was not detected at 1 h (<10 CFU ml 1 ) but grew afterwards, though at lower rates than without phage. For non-O157:H7 STEC, reductions lower than 1 log CFU ml 1 were observed for all sampling times. Phages DT1 and DT6, either individually or as a cocktail, effectively reduce O157:H7 STEC counts during milk fermentation, without compromising the starter culture performance.
dc.format application/pdf
dc.language.iso eng
dc.publisher Wiley
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject ESCHERICHIA COLI O157:H7 STEC
dc.subject BACTERIOPHAGE
dc.subject PHAGE BIOCONTROL
dc.subject MILK FERMENTATION
dc.subject.classification Otros Tópicos Biológicos
dc.subject.classification Ciencias Biológicas
dc.subject.classification CIENCIAS NATURALES Y EXACTAS
dc.title Phage biocontrol of enteropathogenic and Shiga toxin-producing Escherichia coli during milk fermentation
dc.type info:eu-repo/semantics/article
dc.type info:ar-repo/semantics/artículo
dc.type info:eu-repo/semantics/publishedVersion
dc.date.updated 2017-07-24T12:50:19Z
dc.journal.volume 57
dc.journal.number 1
dc.journal.pagination 3-10
dc.journal.pais Estados Unidos
dc.journal.ciudad Hoboken
dc.description.fil Fil: Tomat, David Damian. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil Fil: Mercanti, Diego Javier. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil Fil: Balague, Claudia Elisabeht. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
dc.description.fil Fil: Quiberoni, Andrea del Lujan. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.journal.title Letters In Applied Microbiology
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1111/lam.12074
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/lam.12074/abstract


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info:eu-repo/semantics/restrictedAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)