Mostrar el registro sencillo del ítem
dc.contributor.author
Garzón, Antonela Guadalupe
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.contributor.author
Veras, Flávio F.
dc.contributor.author
Brandelli, Adriano
dc.contributor.author
Drago, Silvina Rosa
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.date.available
2023-10-23T15:56:04Z
dc.date.issued
2022-01
dc.identifier.citation
Garzón, Antonela Guadalupe; Veras, Flávio F.; Brandelli, Adriano; Drago, Silvina Rosa; Purification, identification and in silico studies of antioxidant, antidiabetogenic and antibacterial peptides obtained from sorghum spent grain hydrolysate; Elsevier Science; LWT - Food Science and Technology; 153; 1-2022; 1-9
dc.identifier.issn
0023-6438
dc.identifier.uri
http://hdl.handle.net/11336/215661
dc.description.abstract
The identification of antioxidant, antidiabetogenic and antimicrobial peptides obtained from sorghum spent grain (SSG) protein hydrolysate was performed. Antioxidant activity (AOA) was evaluated by ABTS+ radical cation scavenging, antidiabetogenic activity (ADA) by dipeptidyl peptidase IV (DPP-IV) inhibition, and antimicrobial activity (AMA) through the minimum inhibitory concentration (MIC) method against 2 Gram-negative and 3 Gram-positive bacteria. SSG peptides were fractionated using a Sephadex G-25 molecular exclusion column, and fractions collected (F1–F6) were evaluated through AOA, ADA and AMA assays. The chromatographic process allowed increasing 20% the AOA and 35% the ADA in F2, and 90% the AMA in fractions F5 and F6. The most active fractions (F2, F5 and F6) were evaluated using LC-ESI-Q-TOF tandem mass spectrometry, which allowed identifying six new peptides with high bioactivity. Moreover, the docking refinement protocol showed that identified peptides could interact with the DPP-IV enzyme, two of them in a competitive inhibition mode. Sorghum spent grain (SSG) could be an important source of bioactive peptides. Additionally, this is the first report indicating the presence of antibacterial peptides encrypted in sorghum proteins.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Science
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.subject
BIOACTIVE PEPTIDES
dc.subject
DOCKING
dc.subject
FRACTIONATION
dc.subject
SORGHUM
dc.subject
TANDEM MASS SPECTROMETRY
dc.subject.classification
Alimentos y Bebidas
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.subject.classification
Otras Ingenierías y Tecnologías
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.subject.classification
INGENIERÍAS Y TECNOLOGÍAS
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.title
Purification, identification and in silico studies of antioxidant, antidiabetogenic and antibacterial peptides obtained from sorghum spent grain hydrolysate
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2023-10-19T14:33:22Z
dc.journal.volume
153
dc.journal.pagination
1-9
dc.journal.pais
Países Bajos
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Garzón, Antonela Guadalupe. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Tecnología de los Alimentos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
dc.description.fil
Fil: Veras, Flávio F.. Universidade Federal do Rio Grande do Sul; Brasil
dc.description.fil
Fil: Brandelli, Adriano. Universidade Federal do Rio Grande do Sul; Brasil
dc.description.fil
Fil: Drago, Silvina Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Tecnología de los Alimentos; Argentina
dc.journal.title
LWT - Food Science and Technology
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S002364382101567X
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.lwt.2021.112414
Archivos asociados