Evento
ATP and adenosine modulate acetylcholine release through P2Y and P1 receptors at The efferent-inner hair cell synapse in the developing inner ear
Tipo del evento:
Reunión
Nombre del evento:
XXIX Annual Meeting and SAN-ISN small conference and course
Fecha del evento:
29/09/2014
Institución Organizadora:
Sociedad Argentina de Investigación en Neurosciencias;
Título del Libro:
XXIX Annual Meeting and SAN-ISN small conference and course
Título de la revista:
XXIX Congreso Anual de la Sociedad Argentina de Investigación en Neurociencias (SAN)
Editorial:
Sociedad Argentina de Investigación en Neurociencias
Idioma:
Inglés
Clasificación temática:
Resumen
Before the onset of hearing (postnatal day 12 in mice) inner hair cells (IHCs) are transiently innervated by medial olivocochlear (MOC) efferent fibers. Acetylcholine (ACh) released by these fibers activates alpha9alpha10 nicotinic receptors coupled to SK2 calcium-activated potassium channels, leading to inhibitory post synaptic currents (IPSCs). During this period, IHCs fire spontaneous sensory-independent action potentials that are required for normal development of the auditory pathway. This activity is driven and/or modulated by ATP released from cochlear supporting cells. ACh release from efferent fibers also contributes to this modulation. By electrically stimulating MOC fibers and recording IPSCs, we showed that ATP decreases ACh release in a reversible and concentration-dependent manner. In this work, we demonstrate that this effect is through P2Y receptor activation, as the specific P2Y agonist 2-MeSADP mimicked the effect of ATP. Moreover, the non-hydrolyzable ATP analog ATPgammaS decreased ACh release as well, indicating that this modulation is driven by ATP itself. We further tested if adenosine can also modulate ACh release. Adenosine reversibly decreased the quantal content. CGS15943, a specific P1 receptor antagonist, abolished the effect of adenosine. On the other hand NECA, a specific P1 agonist, decreased ACh release. Our results suggest that both ATP and adenosine inhibit ACh release at the MOC-IHC synapse through the activation of P2Y and P1 receptors, respectively.
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Eventos(INGEBI)
Eventos de INST.DE INVEST.EN ING.GENETICA Y BIOL.MOLECULAR "DR. HECTOR N TORRES"
Eventos de INST.DE INVEST.EN ING.GENETICA Y BIOL.MOLECULAR "DR. HECTOR N TORRES"
Citación
ATP and adenosine modulate acetylcholine release through P2Y and P1 receptors at The efferent-inner hair cell synapse in the developing inner ear; XXIX Annual Meeting and SAN-ISN small conference and course; Huerta Grande; Argentina; 2014; 1-1
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