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dc.contributor.author
Fernandez Chiappe, Florencia  
dc.contributor.author
Muraro, Nara Ines  
dc.date.available
2023-10-20T16:09:59Z  
dc.date.issued
2022-08  
dc.identifier.citation
Fernandez Chiappe, Florencia; Muraro, Nara Ines; Dissection of Drosophila Adult Brains for Patch-Clamping Neurons; Cold Spring Harbor Laboratory Press; Cold Spring Harbor Protocols; 2022; 8; 8-2022; 385-391  
dc.identifier.issn
1940-3402  
dc.identifier.uri
http://hdl.handle.net/11336/215590  
dc.description.abstract
The brain of adult flies (Drosophila melanogaster) has been studied in detail from several perspectives, including the anatomical and molecular characterization of hundreds of neuronal types. However, information regarding the electrophysiological properties of most neuronal types is lacking. This protocol provides detailed information on how to dissect the brain of adult flies to produce an ex vivo preparation in which central neurons can be patch-clamped. Immobilizing fresh and tiny tissues, such as fly brains, to perform successful patch-clamp recordings is a critical step; here, we explain how this can be achieved using cyanoacrylate glue.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Cold Spring Harbor Laboratory Press  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
DROSOPHILA MELANOGASTER  
dc.subject
ELECTROPHYSIOLOGY  
dc.subject
PATCH-CLAMP  
dc.subject
NEUROSCIENCE  
dc.subject.classification
Biología  
dc.subject.classification
Ciencias Biológicas  
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS  
dc.title
Dissection of Drosophila Adult Brains for Patch-Clamping Neurons  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2023-07-10T11:39:38Z  
dc.identifier.eissn
1559-6095  
dc.journal.volume
2022  
dc.journal.number
8  
dc.journal.pagination
385-391  
dc.journal.pais
Estados Unidos  
dc.description.fil
Fil: Fernandez Chiappe, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigación en Biomedicina de Buenos Aires - Instituto Partner de la Sociedad Max Planck; Argentina  
dc.description.fil
Fil: Muraro, Nara Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigación en Biomedicina de Buenos Aires - Instituto Partner de la Sociedad Max Planck; Argentina  
dc.journal.title
Cold Spring Harbor Protocols  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://cshprotocols.cshlp.org/content/2022/8/pdb.prot107935.long  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1101/pdb.prot107935  

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