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dc.contributor.author
Vásquez, Elena
dc.contributor.author
Muñoz, Estela Maris
dc.date.available
2023-10-18T15:55:02Z
dc.date.issued
2019
dc.identifier.citation
Dynamics of GABABR and associated proteins in the postnatal rat cerebellum; XXXIII Congress of the Argentine Society for Research in Neuroscience; Córdoba; Argentina; 2018; 76-76
dc.identifier.issn
1759-0914
dc.identifier.uri
http://hdl.handle.net/11336/215396
dc.description.abstract
Before glutamatergic synapses are formed, GABA-mediated signaling is considered to drive cell differentiation in the developing central nervous system (CNS). GABA, a classical inhibitory neurotransmitter, can also depolarize immature cells. Although this shift is mediated by the ionotropic GABA A receptor (GABAAR), recent evidence suggests that the electrical properties of GABAARs can be modulated by the metabotropic GABA B receptor (GABABR). GABABRs are macromolecular complexes, formed by a G protein-coupled receptor and a large number of constituents that interact together and ultimately influence cell identity and behavior. The composition of these complexes exhibits wide spatiotemporal variations; however, the implications of such dynamism during development of the CNS are far from being understood. We have determined total protein expression of some constituents of GABABRs (GABABR1a; GABABR1b, GABABR2; KCTD12) in the developing cerebellum of postnatal rats at 5, 15 and 90 days after birth, by performing Western Blots. Our findings suggest that the expression levels of the core and auxiliary subunits of GABABRs vary ontogenetically. This dynamism was also observed at the mRNA levels by RT-PCR. In addition, multiple immunolabeling followed by confocal microscopy of cerebellar sections showed Purkinje cells as the most dynamic cell type in terms of subcellular localization of the different molecules studied here. Our data support a cell lineage-dependent GABABR regulation.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
SAGE Publications
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc/2.5/ar/
dc.subject
GABABR
dc.subject
POSNATAL DEVELOPMENT
dc.subject
RAT
dc.subject
CEREBELLUM
dc.subject.classification
Biología del Desarrollo
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Dynamics of GABABR and associated proteins in the postnatal rat cerebellum
dc.type
info:eu-repo/semantics/publishedVersion
dc.type
info:eu-repo/semantics/conferenceObject
dc.type
info:ar-repo/semantics/documento de conferencia
dc.date.updated
2023-02-23T14:00:25Z
dc.journal.volume
11
dc.journal.pagination
76-76
dc.journal.pais
Reino Unido
dc.journal.ciudad
Londres
dc.description.fil
Fil: Vásquez, Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
dc.description.fil
Fil: Muñoz, Estela Maris. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://journals.sagepub.com/doi/10.1177/1759091419834821
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1177/1759091419834821
dc.conicet.rol
Autor
dc.conicet.rol
Autor
dc.coverage
Internacional
dc.type.subtype
Congreso
dc.description.nombreEvento
XXXIII Congress of the Argentine Society for Research in Neuroscience
dc.date.evento
2018-10-24
dc.description.ciudadEvento
Córdoba
dc.description.paisEvento
Argentina
dc.type.publicacion
Journal
dc.description.institucionOrganizadora
Sociedad Argentina de Investigación en Neurociencias
dc.source.revista
American Society for Neurochemistry
dc.date.eventoHasta
2018-10-26
dc.type
Congreso
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