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dc.contributor.author
Carnevale, Matías Emanuel
dc.contributor.author
Balaban, Cecilia Lucía
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Guibert, Edgardo Elvio
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Bottai, Hebe
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Rodriguez, Joaquin Valentin
dc.date.available
2017-07-28T17:52:53Z
dc.date.issued
2013-11
dc.identifier.citation
Carnevale, Matías Emanuel; Balaban, Cecilia Lucía; Guibert, Edgardo Elvio; Bottai, Hebe; Rodriguez, Joaquin Valentin; Hypothermic Machine Perfusion Versus Cold Storage in the Rescuing of Livers From Non-Heart-Beating Donor Rats; Wiley; Artificial Organs; 37; 11; 11-2013; 985-991
dc.identifier.issn
0160-564X
dc.identifier.uri
http://hdl.handle.net/11336/21525
dc.description.abstract
The aim of this work was to compare the effi- ciency of cold storage (CS) and hypothermic machine perfusion (HMP) methods of preserving grafts excised from non-heart-beating donors that had suffered 45 minutes of warm ischemia. We developed a new solution for HMP to use in liver transplantation, based on BES, gluconate, and polyethylene glycol (BGP-HMP solution). After 24 h of HMP or CS, livers were reperfused at 37°C with Krebs–Henseleit solution with added dextran. For both procedures, portal pressure and flow were measured and the intrahepatic resistance (IR) was calculated. The pH oscillations and enzyme activities (LDH, AST, and ALT) were evaluated for the perfusion buffer during normothermic reperfusion. O2 consumption of the liver, glycogen production, and bile flow were also measured during the normothermic reperfusion period. Portal flow and IR showed statistical differences (P < 0.05) between the two groups (n = 5). HMP with BGP-HMP solution resulted in higher values of portal flow and lower IR than CS with HTK solution. Enzyme release after 90 min of reperfusion did not show statistical differences between groups. With regard to bile flow and O2 consumption, livers preserved by both processes were able to produce bile, but livers preserved with HMP were able to take up more O2 than livers preserved by CS.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Wiley
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Hypothermic Machine Perfusion
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Liver Cold Storage
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Non-Heart Beating Donors
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Otros Tópicos Biológicos
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Hypothermic Machine Perfusion Versus Cold Storage in the Rescuing of Livers From Non-Heart-Beating Donor Rats
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-07-26T17:20:06Z
dc.journal.volume
37
dc.journal.number
11
dc.journal.pagination
985-991
dc.journal.pais
Estados Unidos
dc.journal.ciudad
Hoboken
dc.description.fil
Fil: Carnevale, Matías Emanuel. Universidad Nacional de Rosario. Secretaria de Ciencia y Tecnica. Centro Binacional de Investigación en Criobiologia Clinica y Aplicada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Balaban, Cecilia Lucía. Universidad Nacional de Rosario. Secretaria de Ciencia y Tecnica. Centro Binacional de Investigación en Criobiologia Clinica y Aplicada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Guibert, Edgardo Elvio. Universidad Nacional de Rosario. Secretaria de Ciencia y Tecnica. Centro Binacional de Investigación en Criobiologia Clinica y Aplicada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Bottai, Hebe. Universidad Nacional de Rosario; Argentina
dc.description.fil
Fil: Rodriguez, Joaquin Valentin. Universidad Nacional de Rosario. Secretaria de Ciencia y Tecnica. Centro Binacional de Investigación en Criobiologia Clinica y Aplicada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.journal.title
Artificial Organs
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1111/aor.12235
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/aor.12235/abstract


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