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dc.contributor.author Carnevale, Matías Emanuel
dc.contributor.author Balaban, Cecilia Lucía
dc.contributor.author Guibert, Edgardo Elvio
dc.contributor.author Bottai, Hebe
dc.contributor.author Rodriguez, Joaquin Valentin
dc.date.available 2017-07-28T17:52:53Z
dc.date.issued 2013-11
dc.identifier.citation Carnevale, Matías Emanuel; Balaban, Cecilia Lucía; Guibert, Edgardo Elvio; Bottai, Hebe; Rodriguez, Joaquin Valentin; Hypothermic Machine Perfusion Versus Cold Storage in the Rescuing of Livers From Non-Heart-Beating Donor Rats; Wiley; Artificial Organs; 37; 11; 11-2013; 985-991
dc.identifier.issn 0160-564X
dc.identifier.uri http://hdl.handle.net/11336/21525
dc.description.abstract The aim of this work was to compare the effi- ciency of cold storage (CS) and hypothermic machine perfusion (HMP) methods of preserving grafts excised from non-heart-beating donors that had suffered 45 minutes of warm ischemia. We developed a new solution for HMP to use in liver transplantation, based on BES, gluconate, and polyethylene glycol (BGP-HMP solution). After 24 h of HMP or CS, livers were reperfused at 37°C with Krebs–Henseleit solution with added dextran. For both procedures, portal pressure and flow were measured and the intrahepatic resistance (IR) was calculated. The pH oscillations and enzyme activities (LDH, AST, and ALT) were evaluated for the perfusion buffer during normothermic reperfusion. O2 consumption of the liver, glycogen production, and bile flow were also measured during the normothermic reperfusion period. Portal flow and IR showed statistical differences (P < 0.05) between the two groups (n = 5). HMP with BGP-HMP solution resulted in higher values of portal flow and lower IR than CS with HTK solution. Enzyme release after 90 min of reperfusion did not show statistical differences between groups. With regard to bile flow and O2 consumption, livers preserved by both processes were able to produce bile, but livers preserved with HMP were able to take up more O2 than livers preserved by CS.
dc.format application/pdf
dc.language.iso eng
dc.publisher Wiley
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject HYPOTHERMIC MACHINE PERFUSION
dc.subject LIVER COLD STORAGE
dc.subject NON-HEART BEATING DONORS
dc.subject.classification Otros Tópicos Biológicos
dc.subject.classification Ciencias Biológicas
dc.subject.classification CIENCIAS NATURALES Y EXACTAS
dc.title Hypothermic Machine Perfusion Versus Cold Storage in the Rescuing of Livers From Non-Heart-Beating Donor Rats
dc.type info:eu-repo/semantics/article
dc.type info:ar-repo/semantics/artículo
dc.type info:eu-repo/semantics/publishedVersion
dc.date.updated 2017-07-26T17:20:06Z
dc.journal.volume 37
dc.journal.number 11
dc.journal.pagination 985-991
dc.journal.pais Estados Unidos
dc.journal.ciudad Hoboken
dc.description.fil Fil: Carnevale, Matías Emanuel. Universidad Nacional de Rosario. Secretaria de Ciencia y Tecnica. Centro Binacional de Investigación en Criobiologia Clinica y Aplicada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil Fil: Balaban, Cecilia Lucía. Universidad Nacional de Rosario. Secretaria de Ciencia y Tecnica. Centro Binacional de Investigación en Criobiologia Clinica y Aplicada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil Fil: Guibert, Edgardo Elvio. Universidad Nacional de Rosario. Secretaria de Ciencia y Tecnica. Centro Binacional de Investigación en Criobiologia Clinica y Aplicada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil Fil: Bottai, Hebe. Universidad Nacional de Rosario; Argentina
dc.description.fil Fil: Rodriguez, Joaquin Valentin. Universidad Nacional de Rosario. Secretaria de Ciencia y Tecnica. Centro Binacional de Investigación en Criobiologia Clinica y Aplicada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.journal.title Artificial Organs
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1111/aor.12235
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/aor.12235/abstract


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info:eu-repo/semantics/restrictedAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)