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dc.contributor.author
Nardelli, Sheila C.
dc.contributor.author
Silmon de Monerri, Natalie C.
dc.contributor.author
Vanagas, Laura
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dc.contributor.author
Wang, Xiaonan
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Tampaki, Zoi
dc.contributor.author
Sullivan, William J.
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Ángel, Sergio Oscar
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dc.contributor.author
Kim, Kami
dc.date.available
2023-10-17T13:48:38Z
dc.date.issued
2022-02
dc.identifier.citation
Nardelli, Sheila C.; Silmon de Monerri, Natalie C.; Vanagas, Laura; Wang, Xiaonan; Tampaki, Zoi; et al.; Genome-wide localization of histone variants in Toxoplasma gondii implicates variant exchange in stage-specific gene expression; BioMed Central; BMC Genomics; 23; 1; 2-2022; 1-17
dc.identifier.issn
1471-2164
dc.identifier.uri
http://hdl.handle.net/11336/215159
dc.description.abstract
Background: Toxoplasma gondii is a protozoan parasite that differentiates from acute tachyzoite stages to latent bradyzoite forms in response to environmental cues that modify the epigenome. We studied the distribution of the histone variants CenH3, H3.3, H2A.X, H2A.Z and H2B.Z, by genome-wide chromatin immunoprecipitation to understand the role of variant histones in developmental transitions of T. gondii parasites. Results: H3.3 and H2A.X were detected in telomere and telomere associated sequences, whereas H3.3, H2A.X and CenH3 were enriched in centromeres. Histones H2A.Z and H2B.Z colocalize with the transcriptional activation mark H3K4me3 in promoter regions surrounding the nucleosome-free region upstream of the transcription start site. The H2B.Z/H2A.Z histone pair also localizes to the gene bodies of genes that are silent but poised for activation, including bradyzoite stage-specific genes. The majority of H2A.X and H2A.Z/H2B.Z loci do not overlap, consistent with variant histones demarcating specific functional regions of chromatin. The extent of enrichment of H2A.Z/H2B.Z (and H3.3 and H2A.X) within the entire gene (5’UTR and gene body) reflects the timing of gene expression during the cell cycle, suggesting that dynamic turnover of H2B.Z/H2A.Z occurs during the tachyzoite cell cycle. Thus, the distribution of the variant histone H2A.Z/H2B.Z dimer defines active and developmentally silenced regions of the T. gondii epigenome including genes that are poised for expression. Conclusions: Histone variants mark functional regions of parasite genomes with the dynamic placement of the H2A.Z/H2B.Z dimer implicated as an evolutionarily conserved regulator of parasite and eukaryotic differentiation.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
BioMed Central
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by/2.5/ar/
dc.subject
APICOMPLEXA
dc.subject
CHIP-SEQ
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EPIGENETIC
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HISTONES
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PARASITES
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TOXOPLASMA
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TRANSCRIPTIONAL REGULATION
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Bioquímica y Biología Molecular
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dc.subject.classification
Ciencias Biológicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
Genome-wide localization of histone variants in Toxoplasma gondii implicates variant exchange in stage-specific gene expression
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2023-10-17T13:12:51Z
dc.journal.volume
23
dc.journal.number
1
dc.journal.pagination
1-17
dc.journal.pais
Reino Unido
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dc.journal.ciudad
Londres
dc.description.fil
Fil: Nardelli, Sheila C.. Albert Einstein College Of Medicine; Estados Unidos
dc.description.fil
Fil: Silmon de Monerri, Natalie C.. Albert Einstein College Of Medicine; Estados Unidos
dc.description.fil
Fil: Vanagas, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
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Fil: Wang, Xiaonan. University of Cambridge; Reino Unido
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Fil: Tampaki, Zoi. Albert Einstein College Of Medicine; Estados Unidos
dc.description.fil
Fil: Sullivan, William J.. Indiana University; Estados Unidos
dc.description.fil
Fil: Ángel, Sergio Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
dc.description.fil
Fil: Kim, Kami. Albert Einstein College Of Medicine; Estados Unidos
dc.journal.title
BMC Genomics
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1186/s12864-022-08338-6
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