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dc.contributor.author
Boero, Luis Ezequiel
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Goutman, Juan Diego
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Elgoyhen, Ana Belen
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Gomez Casati, Maria Eugenia
dc.date.available
2023-10-17T11:37:52Z
dc.date.issued
2015
dc.identifier.citation
Activity of the alpha9alpha10 nAChR inversely correlates with the magnitude of acoustic injury; XXX Annual Meeting and SAN-ISN Small Conference and Course; Mar del Plata; Argentina; 2015; 322-322
dc.identifier.uri
http://hdl.handle.net/11336/215098
dc.description.abstract
Noise induced hearing loss (NIHL) has become a major public health problem. In order to address the role of the efferent olivocochlear system in NIHL we made use of a mouse model in which the alpha9 nicotinic receptor subunit bears a mutation and leads to enhanced medial efferent activity (Chrna9L9T knock-in (KI)) in addition to one lacking the alpha9 subunit of the nicotinic receptor (Chrna9 knockout (KO)).We exposed WT, Chrna9L9T KI and Chrna9 KO mice to loud sounds (1-16 kHz, 100 dB SPL, 1hr) and measured auditory brainstem responses (ABR), which reflect synchronized discharges from neurons along the auditory pathway. We tested outer hair cell function by recording the distortion product otoacoustic emissions (DPOAEs). Large auditory threshold shifts were found one day after exposure in WT and Chrna9 KO mice. However, one week later, thresholds returned to normal in WT, whereas the Chrna9 KO ears did not recover. In contrast, Chrna9L9T KI mice were resistant to the same noise exposure. Finally, we used immunohistochemistry to visualize efferent neurons and found a reduction in the number of terminals after trauma in WT mice. Immunofluorescence against Ctbp2, a protein located in inner hair cell (IHC) ribbon synapse, revealed a decrease in the number of ribbon synapses per IHC after acoustic trauma in WT mice. These findings suggest a key role of alpha9alpha10 nAChRs in the efferent-mediated noise protection.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Sociedad Argentina de Investigación en Neurociencias
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
EFFERENT SYSTEM
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ACOUSTIC TRAUMA
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COCHLEAR HAIR CELLS
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Otras Ciencias Biológicas
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Activity of the alpha9alpha10 nAChR inversely correlates with the magnitude of acoustic injury
dc.type
info:eu-repo/semantics/publishedVersion
dc.type
info:eu-repo/semantics/conferenceObject
dc.type
info:ar-repo/semantics/documento de conferencia
dc.date.updated
2023-09-15T12:50:42Z
dc.journal.pagination
322-322
dc.journal.pais
Argentina
dc.journal.ciudad
Ciudad Autónoma de Buenos Aires
dc.description.fil
Fil: Boero, Luis Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
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Fil: Goutman, Juan Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
dc.description.fil
Fil: Elgoyhen, Ana Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
dc.description.fil
Fil: Gomez Casati, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://saneurociencias.org.ar/wp-content/uploads/2022/01/SAN2015_Mar-del-Plata._libro-de-resumenes.pdf
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Autor
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Autor
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Autor
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Autor
dc.coverage
Internacional
dc.type.subtype
Reunión
dc.description.nombreEvento
XXX Annual Meeting and SAN-ISN Small Conference and Course
dc.date.evento
2015-09-27
dc.description.ciudadEvento
Mar del Plata
dc.description.paisEvento
Argentina
dc.type.publicacion
Book
dc.description.institucionOrganizadora
Sociedad Argentina de Investigaciones en Neurociencias
dc.source.libro
XXX Annual Meeting and SAN-ISN Small Conference and Course
dc.source.revista
Sociedad Argentina de Neurociencias
dc.date.eventoHasta
2015-10-01
dc.type
Reunión
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