Evento
Activation of presynaptic GABAB receptors minimize depression and enables sustained transmission at high rate of stimulation in cholinergic olivocochlear-hair cell synapses
Tipo del evento:
Congreso
Nombre del evento:
XXXII Congreso Anual de la Sociedad Argentina de Investigación en Neurociencia
Fecha del evento:
25/09/2017
Institución Organizadora:
Sociedad Argentina de Investigación en Neurociencias;
Título del Libro:
XXXII Congreso Anual de la Sociedad Argentina de Investigación en Neurociencia: Abstract book
Editorial:
Sociedad Argentina de Investigación en Neurociencia
Idioma:
Inglés
Clasificación temática:
Resumen
During development, medial olivocochlear (MOC) neurons transiently innervate cochlear inner hair cells (IHCs). Although acetylcholine (ACh) is the main neurotransmitter at this synapse, an abundant GABA innervation is also present. Electrical stimulation of MOC efferent fibers triggers the release of ACh, but also activates presynaptic GABAB receptors, that in turn reduce the amount of ACh release. GABA-mediated mechanism is through the inhibition of P/Q type Ca2+ channels. We are now studying the consequences of GABAB-mediated inhibition in the short-term plasticity of this synapse. Inhibitory synaptic currents (IPSC) were recorded in IHCs of acutely isolated organs of Corti at P9-P11 while MOC fibers were electrically stimulated. In control condition, 10 pulses applied at high frequency (50 Hz) resulted in a progressive decrease on IPSC amplitudes throughout the train (P10/P1= 0.54). Contrary, specific GABAB agonist, baclofen, increased facilitation rate and eliminated depression at the same frequency (P10/P1= 1). Moreover, application of CGP35348, a GABAB antagonist, produced a bigger depression even at low stimulation frequencies (10Hz). These results suggest that the activation of presynaptic GABAB receptors, minimizes depression and would enable sustained transmission during high-frequency stimulation at the MOC-inner hair cell synapse.
Palabras clave:
GABAB RECEPTOR
,
EFFERENT MODULATION
,
COCHLEA
,
SYNAPTIC PLATICITY
Archivos asociados
Licencia
Identificadores
Colecciones
Eventos(INGEBI)
Eventos de INST.DE INVEST.EN ING.GENETICA Y BIOL.MOLECULAR "DR. HECTOR N TORRES"
Eventos de INST.DE INVEST.EN ING.GENETICA Y BIOL.MOLECULAR "DR. HECTOR N TORRES"
Citación
Activation of presynaptic GABAB receptors minimize depression and enables sustained transmission at high rate of stimulation in cholinergic olivocochlear-hair cell synapses; XXXII Congreso Anual de la Sociedad Argentina de Investigación en Neurociencia; Mar del Plata; Argentina; 2017; 1-1
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