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dc.contributor.author Robert, María Celeste
dc.contributor.author Furlan, Giulia
dc.contributor.author Rosso, Natalia
dc.contributor.author Gambaro, Sabrina Eliana
dc.contributor.author Apitsionak, Faina
dc.contributor.author Vianello, Eleonora
dc.contributor.author Claudio, Tiribelli
dc.contributor.author Gazzin, Silvia
dc.date.available 2017-07-27T18:03:25Z
dc.date.issued 2013-11
dc.identifier.citation Robert, María Celeste; Furlan, Giulia; Rosso, Natalia; Gambaro, Sabrina Eliana; Apitsionak, Faina; et al.; Alterations in the Cell Cycle in the Cerebellum of Hyperbilirubinemic Gunn Rat: A Possible Link with Apoptosis?; Public Library of Science; Plos One; 8; 11; 11-2013; 1-13; e79073
dc.identifier.issn 1932-6203
dc.identifier.uri http://hdl.handle.net/11336/21477
dc.description.abstract Severe hyperbilirubinemia causes neurological damage both in humans and rodents. The hyperbilirubinemic Gunn rat shows a marked cerebellar hypoplasia. More recently bilirubin ability to arrest the cell cycle progression in vascular smooth muscle, tumour cells, and, more importantly, cultured neurons has been demonstrated. However, the involvement of cell cycle perturbation in the development of cerebellar hypoplasia was never investigated before. We explored the effect of sustained spontaneous hyperbilirubinemia on cell cycle progression and apoptosis in whole cerebella dissected from 9 day old Gunn rat by Real Time PCR, Western blot and FACS analysis. The cerebellum of the hyperbilirubinemic Gunn rats exhibits an increased cell cycle arrest in the late G0/G1 phase (p < 0.001), characterized by a decrease in the protein expression of cyclin D1 (15%, p < 0.05), cyclin A/A1 (20 and 30%, p < 0.05 and 0.01, respectively) and cyclin dependent kinases2 (25%, p < 0.001). This was associated with a marked increase in the 18 kDa fragment of cyclin E (67%, p < 0.001) which amplifies the apoptotic pathway. In line with this was the increase of the cleaved form of Poly (ADP-ribose) polymerase (54%, p < 0.01) and active Caspase3 (two fold, p < 0.01). These data indicate that the characteristic cerebellar alteration in this developing brain structure of the hyperbilirubinemic Gunn rat may be partly due to cell cycle perturbation and apoptosis related to the high bilirubin concentration in cerebellar tissue mainly affecting granular cells. These two phenomena might be intimately connected.
dc.format application/pdf
dc.language.iso eng
dc.publisher Public Library of Science
dc.rights info:eu-repo/semantics/openAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject HYPERBILIRUBINEMIA
dc.subject CEREBELLUM
dc.subject CELL CYCLE
dc.subject APOPTOSIS
dc.subject.classification Bioquímica y Biología Molecular
dc.subject.classification Ciencias Biológicas
dc.subject.classification CIENCIAS NATURALES Y EXACTAS
dc.title Alterations in the Cell Cycle in the Cerebellum of Hyperbilirubinemic Gunn Rat: A Possible Link with Apoptosis?
dc.type info:eu-repo/semantics/article
dc.type info:ar-repo/semantics/artículo
dc.type info:eu-repo/semantics/publishedVersion
dc.date.updated 2017-07-26T17:20:52Z
dc.journal.volume 8
dc.journal.number 11
dc.journal.pagination 1-13; e79073
dc.journal.pais Estados Unidos
dc.journal.ciudad San Francisco
dc.description.fil Fil: Robert, María Celeste. Centro Studi Fegato; Italia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil Fil: Furlan, Giulia. Centro Studi Fegato; Italia
dc.description.fil Fil: Rosso, Natalia. Centro Studi Fegato; Italia
dc.description.fil Fil: Gambaro, Sabrina Eliana. Centro Studi Fegato; Italia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil Fil: Apitsionak, Faina. Centro Studi Fegato; Italia
dc.description.fil Fil: Vianello, Eleonora. Centro Studi Fegato; Italia
dc.description.fil Fil: Claudio, Tiribelli. Centro Studi Fegato; Italia. Universita Degli Studi Di Trieste; Italia
dc.description.fil Fil: Gazzin, Silvia. Centro Studi Fegato; Italia
dc.journal.title Plos One
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1371/journal.pone.0079073
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0079073
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3815147/


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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)