Artículo
Formation and nanoscale characterization of asymmetric supported lipid bilayers containing raft-like domains
Vazquez, Romina Florencia
; Ovalle García, Erasmo; Antillón, Armando; Ortega Blake, Iván; Muñoz Garay, Carlos; Maté, Sabina María
![Icon](/themes/CONICETDigital/images/conicet.png)
![Icon](/themes/CONICETDigital/images/conicet.png)
Fecha de publicación:
01/2022
Editorial:
Humana Press
Revista:
Methods in Molecular Biology
ISSN:
1064-3745
ISBN:
978-1-0716-1842-4
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
The development of new strategies for achieving stable asymmetric membrane models has turned interleaflet lipid asymmetry into a topic of major interest. Cyclodextrin-mediated lipid exchange constitutes a simple and versatile method for preparing asymmetric membrane models without the need for sophisticated equipment. Here we describe a protocol for preparing asymmetric supported lipid bilayers mimicking membrane rafts by cyclodextrin-mediated lipid exchange and the main guidelines for obtaining structural information and quantitative measures of their mechanical properties using Atomic force microscopy and Force spectroscopy; two powerful techniques that allow membrane characterization at the nanoscale.
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Articulos(INIBIOLP)
Articulos de INST.DE INVEST.BIOQUIMICAS DE LA PLATA
Articulos de INST.DE INVEST.BIOQUIMICAS DE LA PLATA
Citación
Vazquez, Romina Florencia; Ovalle García, Erasmo; Antillón, Armando; Ortega Blake, Iván; Muñoz Garay, Carlos; et al.; Formation and nanoscale characterization of asymmetric supported lipid bilayers containing raft-like domains; Humana Press; Methods in Molecular Biology; 2402; 1-2022; 243-256
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