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dc.contributor.author
Lores, M.
dc.contributor.author
Cabal, C.
dc.contributor.author
Nascimento O,
dc.contributor.author
Gennaro, Ana Maria
dc.date.available
2017-07-27T15:22:57Z
dc.date.issued
2006-12
dc.identifier.citation
Lores, M.; Cabal, C.; Nascimento O,; Gennaro, Ana Maria; EPR study of the hemoglobin rotational correlation time and microviscosity during the polymerization of hemoglobin S; Springer Wien; Applied Magnetic Resonance; 30; 1; 12-2006; 121-128
dc.identifier.issn
0937-9347
dc.identifier.uri
http://hdl.handle.net/11336/21449
dc.description.abstract
The microviscosity and the protein rotational correlation time are analyzed in samples of hemoglobin A and hemoglobin S with the intracellular concentration at 36ºC and during spontaneous deoxygenation. With this purpose, we use glutathione and carbonmonoxy hemoglobin labeled with 4 maleimido 2,2,6,6-tetramethyl-piperidine-l-oxyl (TEMPO) as probes and 4 maleimido TEMPO bound to the hemoglobin (A and S) as a spin label. The saturation transfer electron paramagnetic resonance experiment showed the sigmoidal behavior, and the increase (about twice) of the hemoglobin rotational correlation time and microviscosity during the polymerization process of hemoglobin S. The delay time determined by this method coincides with that obtained in proton magnetic resonance experiments. These results help to explain the temporal behavior of the proton relaxation times obtained in samples of hemoglobin A and S under the same experimental conditions.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Springer Wien
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject.classification
Física de los Materiales Condensados
dc.subject.classification
Ciencias Físicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
EPR study of the hemoglobin rotational correlation time and microviscosity during the polymerization of hemoglobin S
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-07-26T15:23:16Z
dc.journal.volume
30
dc.journal.number
1
dc.journal.pagination
121-128
dc.journal.pais
Austria
dc.journal.ciudad
Viena
dc.description.fil
Fil: Lores, M.. Universidad de Oriente; Cuba
dc.description.fil
Fil: Cabal, C.. Universidad de Oriente; Cuba
dc.description.fil
Fil: Nascimento O,. Universidade de Sao Paulo; Brasil
dc.description.fil
Fil: Gennaro, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina
dc.journal.title
Applied Magnetic Resonance
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1007/BF03166986
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007/BF03166986
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