Artículo
Identification of a Salmonella ancillary copper detoxification mechanism by a comparative analysis of the genome-wide transcriptional response to copper and zinc excess
Pontel, Lucas Blas
; Scampoli, Nadia Lorena
; Porwollik, Steffen; Checa, Susana Karina
; McClelland, Michael; Soncini, Fernando Carlos
Fecha de publicación:
08/2014
Editorial:
Society For General Microbiology
Revista:
Microbiology-uk
ISSN:
1350-0872
e-ISSN:
1465-2080
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Copper and zinc are essential metal ions, but toxic in excess. Bacteria have evolved different strategies to control their intracellular concentrations, ensuring proper supply while avoiding toxicity, including the induction of metal-specific as well as non-specific mechanisms. We compared the transcriptional profiles of Salmonella Typhimurium after exposure to either copper or zinc ions in both rich and minimal media. Besides metal-specific regulatory networks many global stress-response pathways react to an excess of either of these metal ions. Copper excess affects both zinc and iron homeostasis by inducing transcription of these metal-specific regulons. In addition to the control of zinc-specific regulons, zinc excess affects the Cpx regulon and the sE envelope-stress responses. Finally, novel metal-specific upregulated genes were detected including a new copper-detoxification pathway that involves the siderophore enterobactin and the outer-membrane protein TolC. This work sheds light onto the transcriptional landscape of Salmonella after copper or zinc overload, and discloses a new mechanism of copper detoxification.
Palabras clave:
Transcriptional Regulation
,
Metal Response
,
Salmonella
,
Stress
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Articulos(IBR)
Articulos de INST.DE BIOLOGIA MOLECULAR Y CELULAR DE ROSARIO
Articulos de INST.DE BIOLOGIA MOLECULAR Y CELULAR DE ROSARIO
Citación
Pontel, Lucas Blas; Scampoli, Nadia Lorena; Porwollik, Steffen; Checa, Susana Karina; McClelland, Michael; et al.; Identification of a Salmonella ancillary copper detoxification mechanism by a comparative analysis of the genome-wide transcriptional response to copper and zinc excess; Society For General Microbiology; Microbiology-uk; 160; 8; 8-2014; 1659-1669
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